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I. Purpose and Scope <br /> This plan outlines the bioremediation treatment details associated with the diesel fuel spill that resulted in <br /> -5.500 gallons of diesel being released to the underlying soils as the result of a diesel pump supplying fuel to <br /> a generator mishap. <br /> Colorado Stone Quarries (CSQ) reported on October 16, 2019 to the Department of Reclamation and Mining <br /> Safety(DRMS)and Colorado Department of Public Health and Environment(CDPHE)that a diesel fuel release <br /> occurred on October 12, 2019 from an above ground storage tank, impacting the underlying soils below the <br /> tank and generator. CSQ personnel discovered diesel fuel daylighting within a downgradient sump,-1,200 feet <br /> downgradient.The CDPHE issued the release spill tracking#2019-0587 and noted that DRMS will be the lead <br /> agency on monitoring the remediation. <br /> HRL Compliance Solutions Inc. (HRL) was contracted on October 23, 2019 to perform an initial onsite and <br /> evaluate the impacted area to provide support for initial clean-up in conjunction with remediation, sampling, <br /> waste management and monitoring support. <br /> The following information is to provide details of the treatment process, frequency, contingency plans and <br /> ultimately closure of the diesel impacted material. It should be noted that changes in the project scope may <br /> directly affect the specifics of this treatment plan. <br /> II. Plan Applicability <br /> This plan is applicable to the in-situ bioremediation of the diesel impacted soil in the underlying fill material <br /> and area designated as the "sump basin". This plan is not intended to be used as a ground/surface water <br /> remediation plan and is limited to soil/solid media. <br /> A. Bioremediation Background <br /> Enhanced and augmented aerobic bioremediation technologies are used to accelerate naturally occurring in- <br /> situ remediation of petroleum hydrocarbons by indigenous microorganisms and supplement with additional <br /> hydrocarbon-degrading microbes in the subsurface. Enhanced aerobic bioremediation technologies to be <br /> utilized at this site include the addition of a specifically formulated and engineered Micro-blaze product <br /> targeting diesel range organics (C 12-C 15) through the addition of hydrocarbon-degrading bacteria such as <br /> pseudomonas, bacillus, brevebacterium,and others. In addition to the bacteria,there are other non-toxic trade <br /> secrete compounds that increase the production of biosurfactants to facilitate the reduction of surface tension <br /> and formation of micelles enabling the release of hydrocarbons from soil chemical bonds allowing for microbe <br /> utilization. In addition to utilizing enhanced augmented methodologies, the use of oxygen releasing <br /> compounds such H2O2 to chemically enhance the treatment water will be employed to maintain optimal <br /> aerobic activity. These technologies work by providing a supplemental supply of oxygen to the subsurface, <br /> which becomes available to aerobic, hydrocarbon-degrading bacteria. The stoichiometric ratio of oxygen per <br /> hydrocarbon is 3 M 02 per 1 mole of hydrocarbons. Oxygen is considered by many to be the primary growth- <br /> limiting factor for hydrocarbon degrading bacteria, and it is normally depleted in zones that have been <br /> contaminated with hydrocarbons. By using these technologies. rates of biodegradation of petroleum <br /> hydrocarbons can be increased at least one, and sometimes several, orders of magnitude over naturally- <br /> occurring, non-stimulated rates. <br /> l <br />