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• area streams and ponds were sampled in May 1974 with one sample of each community <br />collected from each sampling site. In November 1974, riffle and pool benthic samples were <br />collected from each site. <br />Benthos was collected by disturbing 2 to 3 ft of stream bottom and collecting dislodged <br />animals and debris in a standard dip net. In swift and shallow reaches, benthos were <br />collected with a Surber sampler. Pond benthos were sampled by using a dip net as a <br />shovel. Samples were placed in polyethylene bags, preserved with 10 to 15 percent <br />formalin, and labeled. <br />Periphyton was collected by scraping natural substrates; samples were preserved in 10 to <br />15 percent FAA (Formalin -Alcohol -Acetic Acid). <br />Each benthos sample was sorted in the laboratory and specimens were identified with the <br />aid of a stereo -zoom dissecting microscope to the lowest practicable taxon. The relative <br />abundance of the identified taxa were calculated according to the following formula: <br />• relative abundance `k = total organisms in taxon x 100 <br />total organisms in sample <br />Periphyton samples were vigorously shaken to fragment clumps and mix algal cells, after <br />which an aliquot of sample was removed and placed under a coverslip. Algal cells and <br />colonies were identified and counted with the aid of a phase -contrast compound microscope. <br />Relative abundance data were calculated for the species present. <br />Sites established in May for biotic sampling on Sage Creek and Dry Creek coincide with <br />those selected for water quality, with the exception of one additional biotic sampling <br />site located on Hubberson Gulch a few hundred yards above its confluence with Dry Creek. <br />Two ponds on the study area were also sampled in May. One, in Section 15, T5N, R88W is a <br />natural lake apparently formed by a very old mud slide. The other pond is the uppermost <br />in a series of beaver ponds located in Section 27, T5N, R88W. Locations of all biotic <br />sampling sites are listed in Table 11-1 and indicated on Exhibit 11-3. <br />Only the lower Sage Creek and lower Dry Creek biotic sites were sampled during the <br />. November (autumn) sampling trip. <br />13 <br />