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Rule 2: Permits <br />Following the data collection at the minimum number of transects, statistics will be run in the field to <br />determine whether sample adequacy is achieved. Sampling will be complete when sample adequacy is <br />achieved or when 50 transects have been sampled. Sample adequacy will be determined using the <br />Snedecor-Cochran sample adequacy formula. The formula is as follows: <br />Nm� = (es) divided by (dx)z <br />where: <br />Nm,,, = the minimum number of observations needed, <br />t = the t -table value for a two -tailed t-test with (n-1) degrees of freedom at the 90 percent confidence <br />level, <br />S2 = the sample variance, <br />D = 0.1 (level of precision for estimate of the mean to be within 10 percent of the actual mean), and <br />X = sample mean. <br />Point -intercept data will be used to calculate the following parameters for each community type in <br />potentially affected areas and reference areas: cover (frequency), relative cover, and diversity. The <br />percent total vegetative cover calculation is as follows: <br />% total vegetative cover = # hits on vegetation x 100 <br />Total # of Points <br />The composition of plant species in each area will be evaluated using the vegetative cover data. The <br />following calculation will be used to determine the relative cover of each species per area: <br />% relative cover of species x = % Cover of Species x <br />% Total Veg. cover <br />Species Diversity <br />Species diversity data will be gathered from the point -intercept cover data collection. Species <br />diversity includes the total number of species hit along the transect. A species list of those plants <br />observed during the field investigation will be developed. <br />Shrub and Tree Density <br />In conjunction with the collection of cover data on the disturbed grassland areas, a 5 meter wide and <br />25 meter long belt transect will be established in designated shrub plots. Designated shrub plots will <br />be identified in disturbed areas with high woody stem density relative to adjacent disturbed <br />grasslands. A minimum of three designated shrub plots will be sampled. Five transects will be sampled <br />from each designated shrub plot, if possible. Smaller or larger transects may be employed within each <br />shrub plot depending on the area to be sampled and specific sampling parameter goals. Woody plant <br />density will be determined by counting each individual woody stems within the 5 x 25 meter belt <br />transect. Woody plant density values will be presented as woody plant stems per transect and the <br />extrapolated value of woody plant stems per acre. Plants that are prone to suckering (e.g. Gamble's <br />oak and mountain mahogany) will be sampled by counting each individual woody stem that falls within <br />TR -20 Page 2.05-72 5-4-2018 <br />