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PART I <br />Page 12 of 47 <br />Permit No.: CO0000213 <br />Effluents and Receiving Water to Freshwater Organisms. Fourth Edition, October 2002 (EPA -821-R-02-013) or the most <br />current edition. <br />The following minimum dilution series should be used: 0% effluent (control), 20%, 40%, 60%, 80%, and 100% effluent. <br />If the permittee uses more dilutions than prescribed, and accelerated testing is to be performed, the same dilution <br />series shall be used in the accelerated testing (if applicable) as was initially used in the failed test. <br />Tests shall be done at the frequency listed in Part I.A. Test results shall be reported along with the Discharge <br />Monitoring Report (DMR) submitted for the end of the reporting period when the sample was taken. (i.e., WET testing <br />results for the calendar quarter ending March 31 shall be reported with the DMR due April 28, etc.) The permittee <br />shall submit all laboratory statistical summary sheets, summaries of the determination of a valid, invalid or inconclusive <br />test, and copies of the chain of custody forms, along with the DMR for the reporting period. <br />If a test is considered invalid, the permittee is required to perform additional testing during the monitoring period to <br />obtain a valid test result. Failure to obtain a valid test result during the monitoring period shall result in a violation of <br />the permit for failure to monitor. <br />b. Violations of the Permit Limit, Failure of One Test Statistical Endpoint and Division Notification <br />A chronic WET test is considered a violation of a permit limitation when both the NOEC and the IC25 are at any effluent <br />concentration less than the IWC. The IWC for this permit has been determined to be 100% effluent. <br />A chronic WET test is considered to have failed one of the two statistical endpoints when either the NOEC or the IC25 <br />are at any effluent concentration less than the IWC. The IWC for this permit has been determined to be 100% effluent. <br />In the event of a permit violation, or when two consecutive reporting periods have resulted in failure of one of the two <br />statistical endpoints (regardless of which statistical endpoints are failed), the permittee must provide written <br />notification to the Division. Such notification should explain whether it was a violation or two consecutive failures of a <br />single endpoint, and must indicate whether accelerated testing or a Toxicity Identification Evaluation or Toxicity <br />Reduction Evaluation (TIE or TRE) is being performed, unless otherwise exempted, in writing, by the Division. <br />Notification must be received by the Division within 14 calendar days of the permittee receiving notice of the WET <br />testing results. <br />c. Automatic Compliance Response <br />The permittee is responsible for implementing the automatic compliance response provisions of this permit when one <br />of the following occurs: <br />• there is a violation of the permit limit (both the NOEC and the IC25 endpoints are less than the applicable IWC) <br />• during a report only period when both the NOEC and the IC25 are at any effluent concentration less than the <br />IWC <br />• two consecutive monitoring periods have resulted in failure of one of the two statistical endpoints (either the <br />IC25 or the NOEC), including during a report only period. This determination is made independently for each <br />test species <br />• the permittee is otherwise informed by the Division that a compliance response is necessary. <br />When one of the above listed events occurs, the following automatic compliance response shall apply. The permittee <br />shall either: <br />• conduct accelerated testing using the single species found to be more sensitive <br />• conduct a Toxicity Identification Evaluation (TIE) or a Toxicity Reduction Evaluation (TRE) investigation as <br />described below. <br />L Accelerated Testing <br />