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Twentymile Coal Company Chronic Whole Effluent Toxicity Report <br />Site 2000-62 2" Quarter 2017 <br />ATI Lot # 060617 a `iabc <br />Summary of Chronic Laboratory Conditions & Chronic WET Testing Parameters <br />Parameter <br />CeHodaphnia dubia <br />Pimephalespromeias <br />Test Type <br />7 -day, chronic, static, renewal <br />7 -day, chronic, static,. renewal <br />Temperature de C) <br />25+f- 1 deg C <br />2.5 +1- 1. deg G <br />Li ht Qua4t . <br />ambient laboratory levels <br />ambient laboratory levels <br />Light Intensity (footeand.le.s <br />50-100 ft -c <br />50-100. ft -c <br />Photoperiod <br />16 hours light] 8 hours dark <br />16 hours li htf 8 hours dant <br />_ Tent Chamber Size <br />40 mL <br />500 mL <br />Test Solution VoEume <br />30 mL <br />300 rnL <br />Renewal of Test Solutions <br />ever 24 -hours <br />eve 24 -hours <br />Age of Test Oqanisms <br />less than 24 -hours <br />fess fhan 24 -hours <br /># of organisms per test chamber <br />1 <br />10 <br />�# of replicate test chambers per dilution <br />10 <br />4 <br />Feeding'Re rrne <br />0.2 mL al ae/YTC per da <br />0.1.5 mL,A. nau lii2xdail <br />Ditution Water <br />AlHFSVV <br />MHFSW <br />Test Duration <br />7-8 days <br />7 -days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling went <br />Sampling Event 2 <br />Sampling Event 3 <br />MHFSW <br />H <br />7.58 <br />7,57 <br />7.83 <br />7.71 <br />Dissolved Oxygen m .IL <br />&28 <br />5.84 <br />6.09 <br />6.x37 <br />Conductivit rnhos/cm <br />4070 <br />41:50 <br />4180 <br />330 <br />Ammonia JT2/L) <br />0.31 <br />0.39 <br />0.22 <br />NO <br />Afkalinit (mg/L <br />235.05 <br />239.015 <br />213.06 <br />59.56 <br />Hardness m ]L <br />2364.55 <br />2436.26 <br />2531.88 <br />91.43 <br />Residual Chlorine (rr►glLj <br />ND <br />ND <br />NO <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />Ic" <br />NOEC <br />Control <br />LOEG <br />Cerioda hnia dubia <br />S Survival <br />X100% <br />100% <br />6 <br />>100°l0 <br />Cerioda hnla dubia <br />1 Reproduction <br />l 56.13% <br />40% <br />Z <br />60% <br />Pime hales promelas <br />7 Survival <br />>100% <br />100% <br />90% -#8, Day 4 <br />>100% <br />Pimephales prornelas <br />3 Growth <br />>100% <br />100% <br />>100°I4 <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />NumberExposed <br />Percent5urvival <br />Mean Average Humber of Neonates . <br />Control <br />10 <br />100% <br />36.5 <br />20% <br />10 <br />100% <br />34.9 <br />40% <br />10 <br />100% <br />32.9 <br />60% <br />10 <br />90%fl - #4, Da. 5 <br />30.3 <br />80% <br />10 <br />90% -#8, Day 4 <br />21.;3 <br />100% <br />1 () <br />&Q°yo - 41, #J, Day 3,41 <br />8. <br />