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PART I <br />Page 16 of 52 <br />Permit No.: CO0000906 <br />The following minimum dilution series should be used: 16%, 32%, 63%, 82%, and 100% effluent. If the <br />permittee uses more dilutions than prescribed, and accelerated testing is to be performed, the same <br />dilution series shall be used in the accelerated testing (if applicable) as was initially used in the failed test. <br />Tests shall be done at the frequency listed in Part I.A.2. Test results shall be reported along with the <br />Discharge Monitoring Report (DMR) submitted for the end of the reporting period when the sample was <br />taken. (i.e., WET testing results for the calendar quarter ending March 31 shall be reported with the DMR <br />due April 28, etc.) The permittee shall submit all laboratory statistical summary sheets, summaries of the <br />determination of a valid, invalid or inconclusive test, and copies of the chain of custody forms, along with <br />the DMR for the reporting period. <br />If a test is considered invalid, the permittee is required to perform additional testing during the monitoring <br />period to obtain a valid test result. Failure to obtain a valid test result during the monitoring period shall <br />result in a violation of the permit for failure to monitor. <br />b. Violations of the Permit Limit, Failure of One Test Statistical Endpoint and Division Notification <br />A chronic WET test is considered a violation of a permit limitation when both the NOEC and the IC25 are at <br />any effluent concentration less than the IWC. The IWC for this permit has been determined to be 63% <br />effluent. <br />A chronic WET test is considered to have failed one of the two statistical endpoints when either the NOEC <br />or the IC25 are at any effluent concentration less than the IWC. The IWC for this permit has been <br />determined to be 63% effluent. <br />In the event of a permit violation, or when two consecutive reporting periods have resulted in failure of <br />one of the two statistical endpoints (regardless of which statistical endpoints are failed), the permittee <br />must provide written notification to the Division. Such notification should explain whether it was a <br />violation or two consecutive failures of a single endpoint, and must indicate whether accelerated testing or <br />a Toxicity Identification Evaluation or Toxicity Reduction Evaluation (TIE or TRE) is being performed, unless <br />otherwise exempted, in writing, by the Division. Notification must be received by the Division within 14 <br />calendar days of the permittee receiving notice of the WET testing results. <br />c. Automatic Compliance Response <br />The permittee is responsible for implementing the automatic compliance response provisions of this permit <br />when one of the following occurs: <br />• there is a violation of the permit limit (both the NOEC and the IC25 endpoints are less than the <br />applicable IWC) <br />• two consecutive monitoring periods have resulted in failure of one of the two statistical endpoints <br />(either the IC25 or the NOEC) <br />• the permittee is otherwise informed by the Division that a compliance response is necessary <br />When one of the above listed events occurs, the following automatic compliance response shall apply. The <br />permittee shall either: <br />• conduct accelerated testing using the single species found to be more sensitive <br />• conduct a Toxicity Identification Evaluation (TIE) or a Toxicity Reduction Evaluation (TRE) <br />investigation as described below. <br />i. Accelerated Testing <br />If accelerated testing is being performed, testing will be at least once every two weeks for up to five <br />tests, running only one test at a time, using only the IC25 statistical endpoint to determine if the test <br />passed or failed at the appropriate IWC. Accelerated testing shall continue until; 1) two consecutive <br />tests fail or three of five tests fail, in which case a pattern of toxicity has been demonstrated or 2) two <br />consecutive tests pass or three of five tests pass, in which case no pattern of toxicity has been found. <br />