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Twentymile Coal Company Chronic Whole Effluent Toxicity Report <br />Site 2000-62 1stQuarter 2017 <br />ATI Lot # 032117-1 abc <br />Summary of Chronic Laboratory Conditions & Chronic WET Testing Parameters <br />Parameter <br />Ceriodaphnia dubia <br />Pimephales promelas <br />Test Type <br />7 -day, chronic, static, renewal <br />7 -day, chronic, static, renewal <br />Temperature de C <br />25 +/ - 1 deg C <br />25 +/ - 1 deg C <br />Light Quality <br />ambient laboratory levels <br />ambient laboratory levels <br />Light Intensity footcandles <br />50-100 ft -c <br />50-100 ft -c <br />Photoperiod <br />16 hours light/ 8 hours dark <br />16 hours light/ 8 hours dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />eve 24 -hours <br />eve 24 -hours <br />Age of Test Organisms <br />less than 24 -hours <br />less than 24 -hours <br /># of organisms per test chamber <br />1 <br />10 <br /># of replicate test chambers per dilution <br />10 <br />4 <br />Feeding Regime <br />0.2 mL al ae/YTC per day <br />0.15 mL A. nau hi 2xdail <br />Dilution Water <br />MHFSW <br />MHFSW <br />Test Duration <br />7-8 days <br />7 -days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling Event 1 <br />Sampling Event 2 <br />Sampling Event 3 <br />MHFSW <br />H <br />7.62 <br />7.70 <br />7.72 <br />7.62 <br />Dissolved Oxygen (mg/L <br />6.64 <br />6.76 <br />6.31 <br />6.57 <br />Conductivity mhos/cm <br />3430 <br />3770 <br />3820 <br />296 <br />Ammonia (mg/L <br />0.14 <br />0.24 <br />0.10 <br />ND <br />Alkalinity (mg/L <br />433.26 <br />462.81 <br />475.99 <br />62.29 <br />Hardness (mg/L <br />2023.03 <br />2278.91 <br />2354.87 <br />93.56 <br />Residual Chlorine mg/L) <br />ND <br />ND <br />ND <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />Ic" <br />NOEC <br />Control <br />LOEC <br />Ceriodaphnia dubia <br />Survival <br />>100% <br />100% <br />6 <br />>100% <br />Ceriodaphnia dubia <br />I Reproduction <br />76.78% <br />60% <br />Z <br />80% <br />Pimephales promelas <br />7 Survival <br />>100% <br />100% <br />100% <br />>100% <br />Pimephales promelas <br />Growth <br />>100% <br />100% <br />>100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Mean Average Number of Neonates <br />Control <br />10 <br />100% <br />30.3 <br />20% <br />10 <br />90% - #8, Day 3 <br />28.5 <br />40% <br />10 <br />100% <br />31.2 <br />60% <br />10 <br />100% <br />27.7 <br />80% <br />10 <br />100% <br />21.6 <br />100% <br />10 <br />90% - #1, Day 5 <br />13.4 <br />