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Twentyinile Coal Company Chronic Whole Effluent Toxicity Report <br />Site 2000-62 4"' Quarter 2016 <br />ATI Lot # 101116-1abc <br />Summary of Chronic Laboratory Conditions & Chronic WET Testing Parameters <br />Parameter <br />Ceriodaphnia dubia = <br />Pime hales pmeles, <br />Test Type _ <br />_7T day. chronic, stabc, renewal <br />7 -da , chronic, static, renewal <br />Temperature (deg C_ <br />_25 +1- 1 deg C <br />25 +1-1 deg C <br />light Quahtty <br />- <br />ambient laboratory levels <br />ambient laboratory levels <br />Light Intensity (footcandles) - <br />_ 50-100 ft -c <br />_ <br />50-100 ft -c <br />__ PhotO�edod <br />16 hours IighU 8 hours dark T <br />16 hours IighU 8 hours dark <br />Test Chamber Size <br />40 mL <br />500 ml <br />est Solution Volume <br />_ 30 mL <br />-- -- <br />� 300 mL <br />_ Renewal of Test Solutions_ <br />_ _ <br />every 24 -hours <br />every 24 -hours <br />Age of Test Organisms <br />_ _ <br />fess than 24 -hours <br />_ <br />less than 24 -hours <br /># of organisms per test chamber <br />—1" ` <br />10 - <br /># of replicate test chambers Qer dilution <br />�10 <br />_ Feedm9 Regime _ <br />_ 0 2 ml algaeMC per day <br />0.15 ml A. nau�r 2xdaily <br />__ - - Dilution Water_ <br />_ <br />MHFSW <br />_- <br />MHFSW <br />Test Duration <br />7-8 da s <br />7 -days .. <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sam lin Event 1 <br />Sam lin Event 2 Sampling Event 3 <br />MHFSW <br />-.___--_ pH ____ <br />7.46 <br />7.61 <br />7 55 <br />7.73 <br />Dissolved Oxygen (mgiL) __ <br />_ 6 41 <br />6.17 <br />6.54 <br />665 <br />Conductivity (umhostcm)- <br />4590 <br />4350 <br />0.06 <br />4430 <br />017 <br />_ <br />306 <br />ND <br />Ammonia (mg1L) ND <br />Alkalinity (rn9/t) <br />4.8 - <br />419.29 n <br />2660.69 <br />ND <br />_ _ 41430- <br />2674 70 <br />ND <br />94.90 <br />ND <br />-______±28.27 __ _ <br />Hardness (mg/L) 2732 78 <br />__ <br />Residual Chlorine iL) ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />S cies Parameter <br />IC <br />NOEC <br />LOEC <br />Cenoda hnia dubia Survival <br />_>100% <br />100%_ <br />>100% <br />Cedode hnia dubia Reproduction <br />57.72% <br />40% <br />60% <br />Pime ales omela Survival <br />>100% <br />_ <br />100°% <br />100°%- <br />>100% <br />>100°% ^ <br />Prmephales promela Growth >100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Mean Average Number of Neonates <br />--Control <br />10 <br />100% <br />286 <br />20%10 <br />_ 100°% <br />-90°%-#5, Rayl <br />100% <br />31.4 _ <br />— w 26.8 _T <br />21.1 <br />40°% - - 10 <br />60% 10 _ <br />80% _ <br />10 <br />80%-#2,#9 <br />100% <br />10 <br />80°% - #4, #9 <br />4.8 - <br />k <br />