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menrymite L oai L;ompany Uhronic Whole Effluent Toxicity Report <br />Site 2000-62 3' Quarter 2016 <br />ATI Lot # 072616-1 abc <br />Summary of Chronic Laboratory Conditions & Chronic WET Testing Parameters <br />Parameter <br />Ceriodaphnia dubia <br />Pimephales promelas <br />Test T <br />7 -day, chronic, static, renewal <br />7 -day, chronic, static, renewal <br />Temperature de C <br />25 +/ -1 deg C <br />25 +/-1 deg C <br />Light Quality <br />ambient laboratory levels <br />ambient laboratory levels <br />Light Intensity footcandles <br />50-100 ft -c <br />50-100 ft -c <br />Photoperiod <br />16 hours light/ 8 hours dark <br />16 hours light( 8 hours dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />eve 24 -hours <br />eve 24 -hours <br />Age of Test Organisms <br />less than 24 -hours <br />less than 24 -hours <br /># of organisms per test chamber <br />1 <br />10 <br /># of replicate test chambers per dilution 1 <br />10 <br />4 <br />Feeding Regime <br />0.2 mL al ae/YTC per day <br />0.15 mL A. nau hi 2xdail <br />Dilution Water <br />MHFSW <br />MHFSW <br />Test Duration <br />7-8 days <br />7 -days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling Event 1 Sampling Event 2 Sampling Event 3 <br />MHFSW <br />H <br />7.39 <br />7.50 <br />7.46 <br />7.64 <br />Dissolved Oxy en (mg/L <br />6.11 <br />5.72 <br />6.29 <br />6.77 <br />Conductivity mhos/cm <br />4580 <br />4560 <br />4590 <br />318 <br />Ammonia (mg/L <br />0.08 <br />0.11 <br />0.04 <br />ND <br />Alkalinity (mg/L <br />368.76 <br />392.75 1 <br />400.74 <br />62.16 <br />Hardness (mg/L <br />2879.79 <br />2865.76 <br />2869.77 <br />98.80 <br />Residual Chlorine m /L) <br />ND <br />ND <br />ND <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />IC, <br />NOEC <br />LOEC <br />Ceriodaphnia dubia <br />Survival — <br />>100% <br />100% <br />6 >100% <br />Ceriodaphnia dubia <br />Reproduction <br />66.49% <br />60% <br />Z 80% <br />Pime hales promela <br />Survival 7 <br />>100% <br />100% <br />>100% <br />Pimephales promelas <br />Growth 2, <br />76.94% <br />60% <br />80% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Mean Average Number of Neonates <br />Control <br />10 <br />100% <br />37.8 <br />20% <br />10 <br />100% <br />35.9 <br />40% <br />10 <br />100% <br />37.2 <br />60% <br />10 <br />100% <br />33.4 <br />80% <br />10 <br />1 90% <br />19.4 <br />100% <br />10 <br />90% <br />9.7 <br />