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Trr entymile Coal Company Chronic 1r hose Effluent Toxicity Report <br />Site 2000-62 2"d Quarter 2016 <br />A;tTi Lot #x 060716-2abe <br />Summary of Cbronic Laboratory Conditions & Chronic WET Testing Parameters <br />Parameter <br />Ceriodaphnia duhia <br />Pimephates promelas <br />Test T pe <br />7 -day, chronic, static, renewal <br />7 -day, chronic,, static, renewal <br />Tem nature (deg C) , <br />25 +/ - 1 deg C <br />25 +1-1 dict G <br />T Light Quality <br />amb_ lent laboratory levets <br />ambient laboratory levels <br />Light lntensity (footcandles) <br />50-100 ft -c <br />50-100 ft -c <br />Photoperiod <br />16 hours G ht/ 8 hours dark <br />16 hours light/ 8 hours dark <br />Test ChamberSize <br />40 mL <br />500 ml- <br />LTest <br />TestSolution volume <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />every 244 tours <br />every 24 -hours <br />Age ofTest Organisms <br />less than 24 -hours <br />less tiian 24 -hours <br /># of organisms per test chamber <br />1 <br />10 <br /># of replicate test chambers per dilution <br />10 <br />4 <br />Feeding Regime <br />0.2 mL ai ae1YTC per day <br />0.15 mL A nau Irl 2xdaii <br />Dilution Water <br />MHFSW <br />MHFSVV <br />Test Duration <br />7-8 days <br />7 -days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling Event 1 <br />Sampling Event 2 Sampling Event 3 <br />MKFSW <br />H <br />7.36 <br />7.33 <br />7.26 <br />7.71 <br />Dissolved Oxygen m /L) <br />5.56 <br />594 <br />5.81 <br />6.65 <br />Conductivity (mhos/cm) <br />4600 <br />4660 <br />4750 <br />311 <br />Ammonia m JL) <br />ND <br />ND <br />0.09 <br />ND <br />Alkahni (m 1L) <br />598.30 <br />593.31 <br />573.33 1 <br />59.73 <br />Hardness (m /L) <br />2986.04 <br />3D61.89 <br />2926.16 <br />98.00 <br />Residual Chlorine (mg/L) <br />ND <br />I ND <br />NO <br />ND <br />Ceriodaphnia dubia & Pimephates promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />ICZs <br />NOEC <br />LOEC <br />Carioda Irma dubia <br />Survival <br />>100% <br />1000/0 <br />t. >100% <br />Ceriodaphnia dubia <br />Reproduction <br />i 47.69% <br />40% <br />60% <br />Pimephales prornelai <br />Survival <br />7 >100% <br />100% <br />i >100% <br />Pimephatesprometa <br />Growth <br />88.72% <br />80% <br />100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Mean Average Number of Neonatos <br />Control <br />10 <br />90% <br />32.4 <br />20% <br />10 <br />100% <br />33.6 <br />40% <br />10 <br />100% <br />28.1 <br />60% <br />10 <br />90% <br />20.8 <br />80% <br />10 <br />80% <br />11.9 <br />100% <br />10 <br />70% <br />0.0 <br />