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S. Wanstedt <br />May 20, 2016 <br />Page 4 <br />hypothesis that TDS (chloride and other TDS ions) is the cause of toxicity. If the mock effluent is <br />much less toxic than the Deserado Mine sample, non-TDS toxicity would be indicated (in addition to <br />chloride). In this case, TIE (Toxicity Identification Evaluation) studies could be conducted to identify <br />the source of toxicity. <br />SUMMARY <br />The results of this PTI suggest that chloride may be the source of toxicity to P. Promelas, although <br />this analysis was based on only two samples. For D. magna, it appears that the level of toxicity <br />observed in a sample from Deserado Mine was somewhat less than what would be expected due to <br />chloride. However, this was based on only one sample. Our recommendation is to measure chloride <br />for all future WET samples, and include a 50% effluent concentration during testing, to determine if <br />there is a relationship between toxicity and chloride. Furthermore, a TDS mock effluent sample could <br />be prepared to assess the role of TDS ions in the observed toxicity. Other TIE procedures could be <br />conducted if any non-TDS toxicity was implicated. <br />Please call one of us if you have any questions about this PTI. We appreciate the opportunity to <br />complete this work for Blue Mountain Energy. <br />Sincerely, <br />"A'a&4,4- <br />Anita B. Rehner, M.S. Rami B. Naddy, Ph.D. <br />QA Manager / Project Manager Manager / Env. Toxicologist <br />rehnerab.tre&mail.com naddyrbe-amail. corn <br />file: 14001-436-023 <br />TRE Environmental Strategies, LLC <br />