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tea 0 ACCO <br />H <br />rl1.44' By . f <br />Report of Toxicity Testing using the Cladoceran Daphnia magna <br />Project ID: 14001-436-017 <br />February 2016 <br />Sponsor and Laboratory Information <br />Test Information <br />Test <br />Blue Mountain Energy, Inc. <br />Sponsor <br />Deserado Mine <br />Test Dates and Time <br />Test Length <br />3607 CR 65 <br />Species <br />Ran el , Colorado 81648 <br />Project Officer <br />Scott Wanstedt 970 675-4322 <br />Outfall <br />TRE Environmental Strategies, LLC <br />Permit Number <br />100 Racquette Drive, Unit A <br />Testing Facility <br />Fort Collins, CO 80524 <br />Concurrent Control Water <br />Fax: (970) 490-2963 <br />Test Concentrations <br />State of Florida NELAP Laboratory ID: E87972 <br />Study Director <br />Rami B. Naddy, Ph.D. (970) 416-0916 email: naddvrb.tret _gmail.com <br />Report Author <br />Peggy Wood (970) 416-0916 email: woodpi.tre0gmail.com <br />Test Information <br />Test <br />Acute Toxicity under Static Conditions' <br />Basis <br />USEPA (2002), method 2021.0 and CDPHE 1998 <br />Test Dates and Time <br />Test Length <br />February 11, 2016 @ 1420 to Februa 13, 2016 0 1410 <br />48 hours <br />Species <br />Daphnia magna <br />Test Material <br />Effluent Grab <br />Outfall <br />Unknown <br />Permit Number <br />CO -0038024 <br />Dilution / Control Water —Receiving <br />Water <br />Concurrent Control Water <br />Moderately Hard Reconstituted Water <br />—(MH), <br />Test Concentrations <br />0 0 Rec Water), 50 and 100% effluent <br />Permit Compliance Pass X Fail Non-compliance <br />n. r1 { . .. 1.. -J.♦L. <br />�u� '"LU VVGO LU wIwuai ule test as static -renewal (renewal of test solutions after 24 hours). However, because <br />of a sample storage problem that rendered the sample unusable on day 1, the test was not renewed. Test <br />conditions did meet USEPA (2002) guidelines. <br />• Results described in this report apply only to the samples submitted to the laboratory and <br />analyzed, as listed in the report <br />• Test results comply with NELAC standards. Reports are intended to be considered in their <br />entirety; TRE is not responsible for consequences arising from use of a partial report <br />• This report contains 4 pages plus 2 appendices <br />ime: environmental Strategies, LLC NELAC Accredited Page 1 of 4 <br />