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Water Systems Engineering, Inc. 785.242.6166 http://www.h2osystems.com <br /> Biological Analysis: <br /> MLGW-ACR Detection Limit <br /> 12:15 <br /> Plate Count (colonies/ml) 2 NA <br /> Anaerobic Growth (%) >10 NA <br /> Sulfate Reducing Bacteria Negative NA <br /> Fe/Mn Oxidizing Bacteria Positive NA <br /> ATP (cells per ml) Initial 1.2 Million NA <br /> ATP (cells per ml) 24 Hour 1.0 Million NA <br /> Total Coliform Negative NA <br /> E.coli Coliform Negative NA <br /> Bacterial Identification Flavobacterium mizutii NA <br /> Bacterial Identification Gallionella NA <br /> Microscopic Evaluation: <br /> MLGW-ACR: Moderate visible bacterial activity, minor amount of crystalline debris, very low <br /> number of protozoa, heavy iron oxide, heavy iron oxide entrained biomass with <br /> moderate number of Gallionella. <br /> Observations and Interpretations: <br /> When received in the lab the sample was amber in color and contained soft amber sediment. <br /> The chemical analyses found low alkalinity with moderate hardness, and total dissolved solids <br /> below a level of concern for a potable water supply. The well displayed a basically neutral pH. <br /> The oxidation-reduction potential (ORP) was indicative of an oxidative condition with a tendency <br /> for metal oxide deposition in the presence of metal ions. The ORP was considerably higher in <br /> the sample when received in the lab than was recorded by field instruments when the sample <br /> was taken. This is not uncommon since the electrochemical properties of a sample are <br /> constantly changing and the two sets of readings were taken approximately two days apart. <br /> The Langelier Saturation Index, which is a calculation of the amount of dissolved calcium <br /> carbonate in the water and an indication of the potential for calcium carbonate mineral <br /> deposition, was negative implying an under saturated condition with little likelihood of carbonate <br /> scale deposition although a negative saturation index in this range is an indication of potentially <br /> corrosive groundwater <br /> Overall there appears to be a low level of dissolved mineral content in the groundwater at this <br /> site. Chemicals and compounds found to be present at concentrations above an ideal <br /> groundwater concentration included potassium, resuspended iron, and total organic carbon. <br /> Resuspended iron accounts for chemically oxidized iron that has become entrapped in biomass <br /> as well as iron that has been mobilized by the activity of iron related bacteria. A high <br /> resuspended iron concentration can indicate the presence of iron related bacteria as well as the <br /> entrapment and accumulation of iron oxide in biomass present within the well system. <br /> 20175.w.pw.p.MLGW-ACR Page 3 of 6 <br />