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Twentymile Coal Company Chronic Whole Effluent Toxicity Report <br />Site -2000-62 3rd Quarter 2015 <br />ATI Lot # 081115-1 abc <br />Summary of Laboratory Conditons & Bioassay Results <br />Parameter <br />corlodaphnla dubfa <br />Pbnepbales promelas <br />Test T e <br />7 -day static renewal <br />7 -day static renewal <br />Temperature de C <br />25+1-1 deg C <br />25+1-1 deg C <br />Light Quali <br />ambient lab levels <br />ambient lab levels <br />Li ht Intensii footcandles <br />60-100 ft -c <br />54-100 ft -0 <br />Photoperiod <br />16 hr li htl8 hr dark <br />16 hr li htf' hr dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />every 24 hours <br />every 24 hours <br />Age of Test Organisms <br />less than 24 hours <br />less than 24 hours <br /># of neonates ear test chamber <br />1 <br />10 <br /># replicate test chambers per cont. <br />10 <br />4 <br />Feeding regime <br />0.2 mL nlaeffTC Ffrelay <br />0.15mLA navO02Wally <br />Dilution WaterMHFSW <br />MHFSW <br />Test Duration <br />7 8 da s <br />?-days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />tf 1✓7'Fi4C1 L 0(1f_)( <br />(a I Y l' C <br />Parameter <br />Sampling Event 1 <br />Sam lin Event 2 <br />Sam lin Event 3 <br />MHFSW <br />H <br />B.06 <br />8.17 <br />7.89 <br />7.81 <br />Dissolved Oxygen (m/L <br />7.06 <br />7.18 <br />7.11 <br />6.72 <br />Conductivi mhoslcm <br />3570 <br />4140 <br />3980 <br />317 <br />Ammonia m 1L <br />ND <br />U6 <br />0.04 <br />ND <br />Alkalinity m 1L <br />290.77 <br />307.97 <br />307.17 <br />51.60 <br />Hardness m 1L <br />1974.09 <br />2061.76 <br />2033.91 <br />95.71 <br />Residual Chlorine m 1L <br />ND <br />ND <br />ND <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />IC25 <br />NOEL <br />LOEC <br />Ceriodaphnia dubia <br />Survival <br />>100% <br />100% <br />>100% <br />Ceriodaphnin dubia <br />Reproduction <br />77.10% - <br />60% <br />80% <br />Pimephalespromelas <br />Survival <br />>100% <br />100% <br />>100% <br />pimephalespromelas <br />Growth <br />>100% <br />100% <br />>100 <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Ex osed <br />Percent Survival <br />Mean Avera a Number of Neonates <br />Control <br />10 <br />90% <br />34.1 <br />20% <br />10 <br />100% <br />36.7 <br />40% <br />10 <br />100% <br />33.0 <br />60% <br />10 <br />100% <br />28.6 <br />80% <br />10 <br />100% <br />15.8 <br />100% <br />1 10 <br />90% <br />7.4 <br />