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CETIS Analytical Report <br />V%P, - SOL o� - -1- <br />Report Date: 05 Oct -1512:29 (p 1 of 2) <br />Test Code: 436-012105-1010-5708 <br />Fathead Minnow 96-h Acute Survival Test <br />vs G%1 Test Stat Critical <br />TRE Environmental Strategies <br />Analysis ID: 13.8241-6913 <br />Endpoint: <br />96h Survival Rate <br />CETIS Version: CETISv1.8.7 <br />Analyzed: 05 Oct -1512:29 <br />Analysis: <br />Nonparametrio-Two Sample <br />Official Results: Yes <br />Batch ID: 03.6199-6933 <br />Test Type: <br />Survival (96h) <br />Analyst: Lab Tech <br />Start Date: 30 Sep -1513:45 <br />Protocol: <br />EPA1821/R-02-012 (2002) <br />Diluent: Mod -Hard Synthetic Water <br />Ending Oats: 04 Oct -1513:15 <br />Species: <br />Plmepha(es promelas <br />Brine: Not Applicable <br />Duration: 96h <br />Source: <br />In -House Culture <br />Age: 4d <br />Sample ID: 08-8145-9336 <br />Code: <br />34BA0088 <br />Client: Blue Mountain Energy <br />Sample Date: 28 Sep -15 08:17 <br />Material: <br />Ambient Sample <br />Project: Special Studies <br />Receive Data: 29 Sep -1513:50 <br />Source: <br />Discharge Monitoring Report <br />0.03665449 0.006109081 <br />Sample Age: 53h (3.3'C) <br />Station: <br />Total <br />Data Transform Zeta <br />All Hyp Trials Seed <br />PMSD Test Result <br />Angular (Corrected) NA <br />C > T <br />NA NA <br />6.92% Fails 96h survival rate <br />Wilcoxon Rank Sum Two -Sample Test <br />Control <br />vs G%1 Test Stat Critical <br />Ties DF P -Value <br />P -Type Decision(a:6%) <br />Dilution Water <br />100• 10 NA <br />0 6 <br />0.0143 <br />Exact Significant Effect <br />Test Acceptability Criteria <br />CV% <br />%Effect <br />0 <br />Attribute <br />Test Stat TAC Limits Overlap <br />Decision <br />1 <br />1 <br />Control Resp <br />1 0.9 - NL Yes <br />Passes Acceptability <br />Criteria <br />ANOVA Table <br />0.0% <br />100 <br />Rep 1 <br />4 <br />Source <br />Sum Squares Mean Square <br />DF <br />F Stat <br />P Value Decialon(a:6%) <br />Between <br />1.595257 1.595257 <br />1 <br />261.1 <br /><0.0001 Significant Effect <br />Error <br />0.03665449 0.006109081 <br />6 <br />Total <br />1.631912 <br />7 <br />C-% <br />Control Type <br />Distributional Tests <br />Mean <br />95% LCL <br />95% UCL <br />Attribute <br />Test Test Stat <br />Critical <br />P Value <br />Decision(0:1%) <br />Variances <br />Mod Levene Equality of Variants 1 <br />13.7 <br />0.3559 <br />Equal Variances <br />Variances <br />Levene Equality of Variance 9 <br />13.7 <br />0.0240 <br />Equal Variances <br />Distribution <br />Shapiro -Wilk W Normality 0.7065 <br />0.645 <br />0.0027 <br />Non -normal Distribution <br />96h Survival Rate Summary <br />C-% <br />Control Type <br />Count <br />Mean <br />961/6 LCL <br />95% UCL <br />Median <br />Min <br />Max <br />Std Err <br />CV% <br />%Effect <br />0 <br />Dilution Water <br />4 <br />1 <br />1 <br />1 <br />1 <br />1 <br />1 <br />0 <br />0.0% <br />0.0% <br />100 <br />Rep 1 <br />4 <br />0.25 <br />0.09088 <br />0.4091 <br />0.2 <br />0.2 <br />0.4 <br />0.05 <br />40.0% <br />75.0% <br />Angular (Corrected) Transformed Summary <br />0.6847 <br />0.4636 <br />C-% <br />Control Type <br />Count <br />Mean <br />95% LCL <br />95% UCL <br />Median <br />Min <br />Max <br />Std Err <br />CV% <br />%Effect <br />0 <br />Dilution Water <br />4 <br />1.412 <br />1.412 <br />1.412 <br />1.412 <br />1,412 <br />1.412 <br />0 <br />0.0% <br />0.0% <br />100 <br />4 <br />0.5189 <br />0.343 <br />0.6948 <br />0.4636 <br />0.4636 <br />0.6847 <br />0.05527 <br />21.3% <br />63.3% <br />96h Survival Rate Detail <br />C!/6 Control Type <br />Rep 1 <br />Rep 2 <br />Rep 3 <br />Rep 4 <br />0 Dilution Water <br />1 <br />1 <br />1 <br />1 <br />100 <br />0.2 <br />0.2 <br />0.4 <br />0.2 <br />Angular (Corrected) Transformed Detail <br />C-%1 Control Type <br />Rep 1 <br />Rep 2 <br />Rep 3 <br />Rep 4 <br />0 Dilution Water <br />1.412 <br />1.412 <br />1.412 <br />1.412 <br />100 <br />0.4636 <br />0.4636 <br />0.6847 <br />0.4636 <br />000 470187-2 CETISTM v1.8.7.16 Analyst: QP -10 /IS <br />