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TRE 14001-436-011 <br />Test Organism <br />Species <br />Daphnia magna <br />Age <br />_ <br /><24 hours <br />Source <br />TRE In-house culture, batch 092915 <br />Acclimation <br />None <br />Feeding <br />None during the test <br />Reference Toxicant Testing <br />Initiated September 9, 2015 using sodium chloride NaCl <br />TEST RESULTS <br />Biological Data <br />° <br />Test Concentration (/o Effluent) <br />Percent Survival of Daphnia magna <br />24 hours 48 hours <br />0 (Control) <br />100 90 <br />100 <br />0 0 <br />Control Performance <br />Acceptable <br />Note: See Appendix B for copies of laboratory data sheets <br />Data Analysis and Test Endpoints <br />Biological Endpoint <br />Statistical Endpoint Value (% Effluent) <br />Survival <br />NOAEC <100 <br />48-hour I-Cso <100 <br />NOAEC = No Observed Adverse Effect Concentration <br />Note: Analyses completed using, where appropriate,CETIS version 1.8.7 (2014). <br />Physical and Chemical Data <br />e Temperature in test solutions <br />"Continuous temperature in water bath <br />TRE Environmental Strategies, LLC NELAC Accredited Page 3 of 4 <br />pH <br />Dissolved <br />Conductivity <br />Temperature <br />Treatment (% Effluent) <br />Oxygen (mglL) <br />(iLSicm) <br />(°C) <br />Low <br />High <br />Low <br />High <br />Low <br />High <br />I Low <br />High <br />0 (Control) <br />8.2 <br />8.4 <br />7.1 <br />7.2 <br />322 <br />358 <br />20 <br />20 <br />100 <br />7.9 <br />8.7 <br />7.2 <br />7.2 <br />16,110 <br />17,150 <br />20 <br />20 <br />208 <br />202 <br />Both Treatments <br />7.9 <br />8.7 <br />'aTi <br />NA <br />19° <br />20° <br />e Temperature in test solutions <br />"Continuous temperature in water bath <br />TRE Environmental Strategies, LLC NELAC Accredited Page 3 of 4 <br />