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tG 1M ACCO., <br />TR17A <br />4 <br />Report of Toxicity Testing using the Cladoceran Daphnia magna <br />Project ID: 14001-436-011 <br />September 2015 <br />Sponsor and Laboratory Information <br />Test Information <br />Test <br />Blue Mountain Energy, Inc. <br />Sponsor <br />Deserado Mine <br />Test Dates and Time <br />3607 CR 65 <br />Test Length <br />Ran el , Colorado 81648 <br />Project Officer <br />Scott Wanstedt 970 675-4322 <br />Test Material <br />TRE Environmental Strategies, LLC <br />Outfall <br />100 Racquette Drive, Unit A <br />Testing Facility <br />Fort Collins, CO 80524 <br />Control Water <br />Fax: (970) 490-2963 <br />Concurrent Control Water <br />State of Florida NELAP Laboratory ID: E87972 <br />Study Director <br />Rami B. Naddy, Ph.D. (970) 416-0916 email: naddyrb.tre(taomaii.com <br />Report Author <br />Ashley Romero, M,S. (970) 416-0916 email: romeroam.tre@,gmail.com <br />Test Information <br />Test <br />Acute Toxicity under Static -Renewal Conditions <br />Basis <br />USEPA(2002),method 2021.0 and CDPHE 1998 <br />Test Dates and Time <br />September 30 2015 @ 1345 to October 2, 2015 @ 1440 <br />Test Length <br />48 hours <br />Species <br />Daphnia magna <br />Test Material <br />Effluent Grab <br />Outfall <br />-I.Lninown- #026 <br />Permit Number <br />CO -0038024 <br />Control Water <br />Moderately Hard Reconstituted Water <br />Concurrent Control Water <br />N/A <br />Test Concentrations <br />0 control and 100% effluent <br />Permit Compliance <br />Pass Fail X Non-compliance <br />• Results described in this report apply only to the samples submitted to the laboratory <br />and analyzed, as listed in the report <br />• Test results comply with NELAC standards. Reports are intended to be considered in <br />their entirety; TRE is not responsible for consequences arising from use of a partial <br />report <br />• This report contains 4 pages plus 2 appendices <br />TRE Environmental Strategies, LLC NELAC Accredited Page 1 of 4 <br />