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SAMPLE ID: 2000-84 <br />CLIENT NAME: Twenty Mile Coal Can <br />ATI LOT #: 041515-1 <br />SAMPLE DATE: 4/1412015 TI <br />TEST START: 04/15/2015 11: OOA <br />TEST FINISH: 04/17/2015 11:OOA <br />OF TEST-, Acute LC5,, 48-hour, static ren+ <br />RATELY HARD FRESH SYNTHETIC DIL <br />•AMMONIA:. ND <br />HARDNESS: 95.11 m <br />ALKALINITY: 55.65 m <br />CHLORINE: ND. <br />-DATE HOURS CONTROL 6.25, <br />4/15/2015 INITIAL <br />411 612 0 1 5 ' 24 <br />4/17/2015 48 <br />MORTALITY <br />SURVIVAL <br />i <br />Aqua-Tox Inc: <br />4880 Robb Stree. ; Suite 8 <br />Wheat Ridge, C ; 80033 <br />(303) 621-4880 www, qua.-toxxom <br />ACUTE TOXICITY REPORT, <br />I Daphnia'ma na <br />TEST RESULT LC >100% <br />1 i 50% MORTALITY TESD : LASS <br />% SURVIVAL 1000 90% <br />11:OOA I ORGANISM: Daphnia magna <br />ORGANISM AG: X24 hours old <br />I D:O.: <br />6.70 mg/y <br />pH: 7,93 <br />CONDUCTIVITY: <br />301 m icro <br />TEMPERATURE: <br />I <br />. <br />20.0 deg <br />� <br />DILUTIONS (%) <br />20.1 <br />19 R 9.r, <br />rn 7s. ann <br />20 <br />201 <br />1 20 1 <br />20 <br />20. <br />20 <br />20 <br />20 <br />20.1 <br />20 <br />20 <br />'20 <br />20 <br />18 <br />20 <br />19 j <br />20 jL <br />20 <br />20 <br />1 20 <br />18 <br />0 <br />1 <br />0 <br />0 <br />0' <br />0 <br />2, <br />100% <br />95% <br />k90% <br />100%, j <br />100%0 <br />100%. <br />90%0. <br />j <br />AILY CHEMISTRY - MIN/MAX <br />CONTROL 6.25,1 <br />CONDUCTIVITY (micros) <br />'D.O. ;(m9/L) <br />pH <br />TEMPS i ATURE (decd C) <br />DILUTIONS (%) <br />12.5 25 5n 7A Inn <br />3011308 <br />.6181624 <br />1 9261939 <br />1551/1560 <br />2790/280 <br />4060/4080 <br />5300/5320 <br />6;7172 <br />6:7R 2 <br />6,817.3 <br />6;.8.17.3.6'.9/7,:4 <br />6.917.4 <br />7.017:4 <br />7.918.3 <br />8.0/8, <br />8:018:4 <br />8.1/8.4 <br />8."1L8:5. <br />8:218=6 <br />8:318.7 <br />20.0120.4. <br />20.0/20.4 • 20.0120.4 <br />20.0120.4` <br />20:0120.4 <br />20:0120.4 <br />20,:0120.4 <br />GENERAL CHEMISTRY - 2000-84 <br />AMMONIA: 0.02 m§/L Wow : 7.03 mg/L <br />HARDNESS: 1,260.75 rr, g1L pK. 8.26 <br />ALKALINITY: 56.05 mgl'L i CONDUCTIVITY: 5,300 micros; <br />CHLORINE: ND j TEMPERATURE: 20:0 deg C <br />75 100%% 100 <br />DITIONAL COMMENTS: <br />Analysis <br />Conducted by: <br />Date: 1 <br />SPEARMAN-KARBER <br />1 <br />1 <br />Reviewed: <br />EPA -821-R-02-012 October 2002 pg.78 <br />