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Twentymile Coal Company Chronic Whole Effluent Toxicity F <br />Site -2000-62 2nd Quarter 2015 <br />ATI Lot # 051915-2abc <br />Summary of Laboratory Conditons & Bioassay Results <br />Parameter <br />Ceriodaphnia dubia <br />Pimephales promelas <br />Test Type <br />7 -day static renewal <br />7 -day static renewal <br />Temperature (deg C) <br />25 +l - 1 deg C <br />25 +/ - 1 deg C <br />Light Quality <br />ambient lab levels <br />ambient lab levels <br />Light Intensity (footcandles) <br />50-100 ft -c <br />50-100 ft -c <br />Photoperiod <br />16 hr light/ 8 hr dark <br />16 hr light/ 8 hr dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />every 24 hours <br />every 24 hours <br />Age of Test Organisms <br />less than 24 hours <br />less than 24 hours <br /># of neonates per test chamber <br />1 <br />10 <br /># replicate test chambers per cone. <br />10 <br />4 <br />Feeding regime <br />0.2 mL algae/YTC per day <br />0.15 mL A. naupin Maily <br />Dilution Water <br />MHFSW <br />MHFSW <br />Test Duration <br />7-8 days <br />7 --days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling Event 1 <br />Sampling Event 2 <br />Sampling Event 3 <br />MHFSW <br />pH <br />7.95 <br />7.98 <br />7.87 <br />7.94 <br />Dissolved Oxygen (mg/L) <br />7.33 <br />7.56 <br />7.13 <br />6.63 <br />Conductivity (pmhos/cm) <br />3580 <br />3620 <br />3680 <br />303 <br />Ammonia (mg/L) <br />0.02 <br />0,03 <br />0.05 <br />ND <br />Alkalinity (mg/L) <br />283.92 <br />297.49 <br />374.96 <br />53.11 <br />Hardness (mg/L) <br />1828.69 <br />1404.38 <br />2035.86 1 <br />93.43 <br />Residual Chlorine (mg/L) <br />ND <br />ND <br />ND I <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />IC25 <br />NOEC <br />LOEC <br />Ceriodaphnia dubia <br />Survival <br />>100% <br />100% <br />>100% <br />Ceriodaphnia dubia <br />Reproduction <br />50.15% <br />40% <br />60% <br />Pimephales promelas <br />Survival <br />>100% <br />1 100% <br />>100% <br />Pimephales promelas <br />Growth <br />94.13% <br />1 $0% <br />100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Mean Average dumber of Neonates <br />Control <br />10 <br />100% <br />38.7 <br />20% <br />10 <br />90% <br />35.3 <br />40% <br />10 <br />100% <br />32.5 <br />60%/n <br />1 10 <br />100% <br />21.1 <br />80% <br />10 <br />80% <br />10.4 <br />100% <br />10 <br />80% <br />2.2 <br />