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2.2.1.7 Whole Effluent Toxicity (WET) Testing No WET testing was conducted during <br />the water year since there was no discharge through any outfall carrying this requirement. <br />A history of WET testing at Deserado Mine follows below: <br />BME started the whole effluent toxicity (WET) testing program beginning in the fourth <br />quarter of 1989. The mine water effluent was found to be toxic to the test species. <br />Accelerated testing was conducted but the toxicity to the species continued. <br />In a meeting with Colorado Water Quality Control Division (WQCD) staff on January 31, <br />1992, the BME staff reiterated the biomonitoring events, and various measures taken to <br />identify the toxicants and to reduce the toxicity. BME took the following steps to identify <br />potential toxicants. <br />• In the early stages of WET testing, the associated H2S in the effluent was <br />deemed to be a potential toxicant. However, a WET testing done by <br />ENSR Consulting in June 1990 after removing H2S by aeration still <br />showed toxicity. This result indicated that some other factor(s) also <br />contributed to the toxicity. <br />In September, 1990, ENSR Consulting was directed to conduct a "toxicity <br />characterization" study to identify the primary cause of the toxicity. This <br />study considered pH effects, volatile organic carbon, metals chelation, <br />aeration, total dissolved solids (TDS) and ammonia. The lab determined <br />high TDS (chlorides) as the primary cause of the toxicity. However, a <br />recent review shows no correlation between LCso values and chloride or <br />TDS levels. <br />BME took the following measures to reduce toxicity of the effluent. <br />• On -site aeration structures were installed for H2S removal before <br />discharging the effluent. <br />• A pilot test was conducted to identify a flocculent for removing high levels <br />of oil and grease from the effluent. <br />• The main water source of the mine was changed from the high TDS( >5000 <br />ppm) alluvial well water to a significantly lower TDS (500 ppm) surface <br />lagoon. <br />11 <br />