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PART I <br />Pap Na t <br />Pemdt Na COG- 5000 <br />testing results for the fast calendar quarter ending March 31 shall be reported with the due April 28.) The results shall <br />be submitted on the Chronic Toxicity Test report form, available from the Division. Copies of time reports are to be <br />submitted to the Division along with the DMR. <br />The permittee shall conduct each chronic WET test in general accordance with methods described in Short Term Methods <br />for Estmating the Chronic J=W of EPA/600/4-89 /001 or the <br />most current edition, except as modified by the most current Division guidance entitled Giuidelines for CondnCtint <br />Whole Effluent Tg3= Tests. The permittee shall conduct such tests using Ceriodaph�ia dubia and fathead minnows. <br />2. Failure of Test and Division Notification <br />A chronic WET test is failed whenever 1) there is a statistically sipifiicant difference in lethality between the control and <br />any effluent concentration less than or equal to the instteam waste concentration (IWC) 2) the ICu, which represents an <br />estimate of the effluent concentration at which 25% of the test organisms demonstrate innbition as reflected by lethality, is <br />at any effluent concentration less than or equal to the IWC. The permittee uarst provide written notification of the failure of <br />WET test to the Division, along with a statement as to whether a Preliminary Toxicity Investigation (PTI)lroxicity <br />Identification Evaluation ( TIE) or accelerated testing is being performed (see Part 12.7.k.). Notification must be received <br />by the Division within 21 calendar days of the demonstration of chronic WET in the routine required test. <br />Demonstration means no later than the last day of the laboratory test. <br />c. Other WET Testing Requirements Applicable to both Chronic and Acute WET Testing <br />1. Automatic Compliance Schedule Upon Failure of Test <br />If a routine chronic WET test is failed, regardless of whether the limit is in effect, the following automatic compliance <br />schedule shall apply. As part of this, the permittee shall either: <br />i. Proceed to conduct the PTYM investigation as described in Part LB.7.c., or <br />I Conduct accelerated testing using the single species found to be more sensitive. <br />If accelerated besting is being performed, the permittee shall provide written notifications of the results within 14 calendar <br />days of completion of the Pattern of Toxicity/No Toxicity demonstration. Testing will be at least once every two weep for <br />up to five tests until; 1) two consecutive bests fail or three of five tests fail, in which case a pattern of toxicity has been <br />demonstrated or 2) two consecutive tests pass or three of five tests pass, in which case no pattern of toxicity has been found. <br />If no pattern of toxicity is found the toxicity episode is considered to be ended and routine testing is to resume. if a patters <br />of toxicity is found, a PTVM investigation is to be performe& If a pattern of toxicity is not demonstrated but a significant <br />level of erratic toxicity is found, the Division may require an increased frequency of routine monitoring or some other <br />modifed approach. <br />Iraff—JAW <br />The results of the PTMU investigation an to be received by the Division within 120 days of the demonstration of chronic <br />WET in the routine test, as defined above, or if accelerated testing is performed, the date the pattern of toxicity is <br />demonstrated. A status report is to be provided to the Division at the 30, 60 and 90 day (points of the PTUM investigation. <br />The Division may extend the time firame for investigation where reasonable jusstififcstiozi exists. A request for an extension <br />must be made in writing and received prior to the 120 day deadline. Such request must include a justification and supportin <br />data for such an extension. <br />The permittee may use the time for investigation to conduct a PTI or move directly into the TIE. A PTI consists of a brief <br />search for possible sources of WET, which might reveal causes of such toxicity and <br />te <br />corrective actions more <br />simply and cost effectively than a formal TIE. If the PTI allows resolution of the WETncident, the TIE need not <br />necessarily be conducted. If, however, WET is not identified or resolved during the PTA, the TIE must be conducted within <br />the allowed 120 day time fi$me. <br />Any permittee that is required to conduct a PT VTIE investigation shall do so in conformjiance with procedures identified in <br />the following documents, or as subsequently updated: 1) Toxicity Identification Evaluation: Characterization of Chronicall <br />Toxic Effluents, Phase I, EPA/600/6- 91/005F May 92, 2) Methods for Aquatic Toxicity, Identification Evaluations, Phase I <br />Toxicity Characterization Procedures, EPA/600 /6- 91/003 Feb. 91 and 3) Methods for Aquatic Toxicity Identification <br />Evaluations, Phase II Toxicity Identification Procedures, EPA/600/3-8&'035 .Feb. 1989. <br />A fourth document in this series is Methods for Aquatic Toxicity Identification Eval»attons, Phase III Toxicity Confnrmatit <br />Procedures, EPA/600/3- 88/036 Feb. 1989. As indicated by the title, this procedure is in tended to confirm that the suspecte< <br />