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�'� ---. <br />Aqua -Tox, Inc. <br />20 <br />4880 Robb Street, Suite 8 <br />20 <br />Wheat Ridge, CO 80033 <br />20 <br />(303) 621 -4880 www aqua- tox.com <br />?Y <br />ACUTE TOXICITY REPORT <br />20 <br />Daphnia magna <br />SAMPLE ID: 2000 -84 <br />TEST RESULT LC50: >100% <br />CLIENT NAME: 20 Mile Coal Company <br />50% MORTALITY TEST: PASS <br />ATI LOT* 080514 -4 <br />% SURVIVAL 100 %: 85% <br />SAMPLE DATE: 8/4/2014 TIME: 10:30A ORGANISM: Daphnia magna <br />TEST START: 08 -05 -14 10:45A <br />ORGANISM AGE: <24 hours old <br />TEST FINISH: 08 -07 -14 10:45A <br />17 <br />TYPE OF TEST: Acute, LC,,,,, 48 -hour, static, renewal moderately hard fresh synthetic dilution water <br />1 <br />1 <br />MODERATELY HARD FRESH SYNTHETIC DILUTION WATER CONSTITUENT <br />AMMONIA: ND <br />D.O.: 6.70 mg /L <br />HARDNESS: 99.30 mg /L <br />pH: 8.03 <br />ALKALINITY: 56.05 mg /L <br />CONDUCTIVITY: 321 micros <br />CHLORINE: ND <br />TEMPERATURE: 20.3 deg C <br />SURVIVAL DATA <br />DILUTIONS ( %) <br />DATE HOURS CONTROL 6.25 <br />12.5 25 50 75 100 <br />8/5/2014 INITIAL <br />8/6/2014 24 <br />8/7/2014 48 <br />MORTALITY <br />% SURVIVAL <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />19 <br />18 <br />20 <br />19 <br />19 <br />20 <br />19 <br />19 <br />17 <br />0 <br />1 <br />1 <br />0 <br />1 <br />1 <br />3 <br />100% <br />95% <br />1 95% <br />100% <br />95% <br />95% <br />85% <br />DAILY CHEMISTRY - (MIN /MAX) DILUTIONS ( %) <br />CONTROL 6.25 12.5 25 50 75 100 <br />CONDUCTIVITY (micros) <br />D O (mg /L) <br />pH <br />TEMPERATURE (deg C) <br />321/330 <br />1074/1088 <br />1856/1871 <br />2590/2610 <br />3340/3370 <br />4110/4130 <br />4890/4920 <br />6.7/7.2 <br />6.7/7.2 <br />6.6/7.2 <br />6.6/72 <br />6.6/7.3 <br />6.5/7.3 <br />6.5/7.3 <br />8.0/8.3 <br />8.018.4 <br />8.0/8.5 <br />8.0/8.6 <br />8.0/8.7 <br />8.0/8.8 <br />8.018.9 <br />197/20.4 <br />19.7120.4 <br />19.7/20.4 <br />19.7/20.4 <br />19.7/204 <br />19.7/20.4 <br />19.7/20.4 <br />GENERAL CHEMISTRY - 2000 -84 <br />AMMONIA: 0.11 mg /L DOinitiai: 6.51 ITIg /L <br />HARDNESS: 100,72 mg /L DOafleraeration: NA <br />ALKALINITY: 1903.91 mg /L pH: 7.99 <br />CHLORINE: ND CONDUCTIVITY: 4890 micros <br />NOEC LOEC CALCULATION METHOD METHOD REFERENCE <br />75% 100% SPEARMAN - KARBER EPA - 821 -R -02 -012 October 2002 pg.78 <br />ADDITIONAL COMMENTS: <br />Analysis 1 P/ <br />Conducted by: ;(.V,t,1_ Reviewed by: <br />Date: v.,` _....� , Date: �9471 <br />V <br />