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Twentymile Coal Company - Site 62 Chronic WET Results <br />3rd Quarter 2014 ATI Lot # 080514 -1 abc <br />Summary of Laboratory Conditons & Bioassay Results <br />Parameter <br />Ceriodaphnia dubia <br />Pimephales promelas <br />Test Type <br />7 -day static renewal <br />7 -day static renewal <br />Temperature (de C ) <br />25 +/ - 1 deg C <br />25 +/ - 1 deg C <br />Light Quality <br />ambient lab levels <br />ambient lab levels <br />Light Intensity (footcandles) <br />50 -100 ft -c <br />50 -100 ft -c <br />Photo period <br />16 hr light/ 8 hr dark <br />16 hr light/ 8 hr dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />every 24 hours <br />every 24 hours <br />Age of Test Organisms <br />less than 24 hours <br />less than 24 hours <br /># of neonates per test chamber <br />1 <br />10 <br /># replicate test chambers per cons. <br />10 <br />4 <br />Feeding regime <br />0.2 mL algae /YTC per day <br />0.15 mL A naupl/i 2xdaily <br />Dilution Water <br />MHFSW <br />MHFSW <br />Test Duration <br />7 -8 days <br />7 -days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling Event 1 <br />Sampling Event 2 <br />Sampling Event 3 <br />MHFSW <br />pH <br />7.81 <br />7.84 <br />7.87 <br />8.03 <br />Dissolved Oxygen (m /L) <br />6.43 <br />645 <br />6.52 <br />6.67 <br />Conductivity ( mhos/cm) <br />4350 <br />4090 <br />4210 <br />321 <br />Ammonia (m /L) <br />0.02 <br />0.02 <br />0.04 <br />ND <br />Alkalinity (m /L) <br />288.01 <br />289.60 <br />1 286.41 <br />56.05 <br />Hardness (m /L) <br />2558.57 <br />2266.28 <br />2330.34 <br />99.30 <br />Residual Chlorine (mg /L) <br />ND <br />ND <br />ND <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />IC25 <br />NOEC <br />LOEC <br />Ceriodaphnia dubia <br />Survival <br />Sj >100% <br />100% 1, <br />>100% <br />Ceriodaphnia dubia <br />Reproduction <br />43.84% <br />25% <br />z i 50% <br />Pimephales promelas <br />Survival <br />>100% <br />100% <br />re,-) >100% <br />Pimephales promelas <br />Growth <br />3 >100% <br />100% <br />:F'. >100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Average Number of Neonates <br />Control <br />10 <br />100% <br />38.4 <br />12.5% <br />10 <br />100% <br />37.6 <br />25% <br />10 <br />100% <br />34.0 <br />50% <br />10 <br />100% <br />27.1 <br />75% <br />10 <br />100% <br />22.3 <br />100% <br />10 <br />90% <br />- - - - - -- 9.9 - - - - - -- <br />