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Where: <br />a = total of species density values for life forms of the reclaimed area <br />40 b = total of species density values for the life forms of the relevant reference area <br />c = the sum of the smaller of the species density values for life forms of the reclaimed area and the <br />reference area <br />Only species density of vascular plants will be included. Life forms to be used will include: <br />Annualfbiennial forbs <br />Annual grasses <br />Perennial forbs /Sub - shrubs <br />Perennial cool season grasses <br />Woody plants are not included because they are addressed by separate standards. <br />Alternative Test D: Assessment of the Presence of Native Species - The third alternative, assessment of <br />the species diversity of the reclaimed lands, makes direct reference to the presence of native western North <br />American plant species in the reclaimed area. <br />Total cumulative number of reclaimed area native species ? avg. reference area native species <br />density (number of species /100 square meters) <br />Comparing the cumulative number of species in what, would often be twenty 100 square meters samples in the <br />reclaimed area to the average density in the reference areas actually compares a native species density per 2,000 <br />square meters in the developing revegetated area to the native species density per 100 square meters in the long <br />established native areas. As such, it allows a forward - looking assessment of the developing presence of native <br />40 species without requiring the extended time necessary for these species to actually "fill -in" and actually achieve a <br />density per random 100 square meters equal to that occurring in the undisturbed areas. <br />Shrub Evaluation - Shrub density sampling will be conducted in the reclaimed area using the belt transect method <br />in accordance with Section 4.15.11(c)(i). Belt transects are 2 meters x 50meters in dimension and are randomly <br />oriented from the sample point origin. <br />Sample adequacy (15 minimum samples) for the belt transect method will be evaluated based on the following <br />expression: <br />t 2 s 2 <br />n,, Fd �xl Y <br />Where: <br />nm = Minimum Sample Size <br />s = Sample variance (n -1 degrees of freedom) <br />d =Precision (0.10 for cover and production; 0.15 for woody plant density) <br />x =Sample arithmetic mean <br />t = The (a =.10) t -table value for a 1 tail t -test, n -1 degrees of freedom (infinite degrees of freedom may be <br />used if n >30) <br />n =Sample size <br />This hypothesis will be tested using methods set forth in Section 4.15.11(2). Hypothesis testing may include the <br />null or reverse null approach previously referenced or methods described in 4.15.11(3), where large heterogeneity <br />in shrub density causes large sample sizes and sample variation. <br />RN08 -05 2.05 -117.2 03/12/10 <br />