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Tiwentymile Coal Company •- Site 1 S Chronic Whole Effluent Toxicity Results <br />2nd Quarter 2014 ATI Lot # 051314 -2abc <br />Summary of Laboratory Conditons & Bioassay Results <br />Parameter <br />Ceriodaphnia dubia <br />Pimephales promelas <br />Test Type <br />7tday static renewal <br />7 -day static renewal <br />Temperature (de C) <br />125+/- 1 de C <br />25 +/ - 1 deg C <br />Light Quality <br />ambient lab levels <br />ambient lab levels <br />Light Intensity (footcandles <br />50 -100 ft -c <br />50 -100 ft -c <br />Photoperiod <br />16 <br />hr light/ 8 hr dark <br />16 hr light/ 8 hr dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />.every 24 hours <br />every 24 hours <br />Age of Test Organisms <br />less than 24 hours <br />less than 24 hours <br /># of neonates per test chamber <br />1 <br />10 <br /># replicate test chambers per cone. <br />10 <br />4 <br />Feeding regime <br />0.R mL algaeNTC per day <br />0.15 mL A nauplu 2xdaily <br />Dilution Water <br />MHFSW <br />MHFSW <br />Test Duration <br />7 -8 days <br />7 -days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling Event <br />Sampling Event 2 <br />Sampling Event 3 <br />MHFSW <br />pH <br />_1 <br />I 7.40 <br />7.53 <br />7.33 <br />8.10 <br />Dissolved Oxygen (m /L) <br />5.73 <br />6.56 <br />5.33 <br />6.91 <br />Conductivity ( mhos/cm ) <br />2240 <br />2260 <br />2260 <br />314 <br />Ammonia (mg/L) <br />0.05 <br />0.06 <br />0.04 <br />ND <br />Alkalinity (mg /L) <br />527.78 <br />499.74 <br />551.82 <br />57.69 <br />Hardness (mg /L) <br />853.58 <br />718.64 <br />756.62 <br />97.75 <br />Residual Chlorine (mg /L) <br />ND <br />ND <br />ND <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />IC25 <br />NOEC <br />LOEC <br />Ceriodaphnia dubia <br />Survival <br />! >100% <br />100% <br />>100% <br />Ceriodaphnia dubia <br />Reproduction <br />>100% <br />100% <br />>100% <br />Pimephales promelas <br />Survival <br />>100% <br />100% <br />>100% <br />Pmephales promelas <br />Growth <br />>100% <br />100% <br />>100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Average Number of Neonates <br />Control <br />10 <br />100% <br />34.3 <br />12.5% <br />10 <br />100% <br />37.8 <br />25% <br />10 <br />100% <br />35.0 <br />50% <br />10 <br />100% <br />36.1 <br />75% <br />10 <br />100% <br />35.4 <br />100% <br />10 <br />100% <br />36.8 <br />