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Twentymile Coal Company -, Site 62 Chronic Whole Effluent Toxicity Results <br />2nd Quarter 2014 ATI Lot # 051314 -3abc <br />Summary of Laboratory Conditons & Bioassay Results <br />Parameter <br />Qeriodaphnia <br />dubia <br />Pimephales promelas <br />Test Type <br />7�day <br />static renewal <br />7 -day static renewal <br />Temperature (de C) <br />8.11 <br />' 5 +/ - 1 deg C <br />25 +/ - 1 deg C <br />Light Quality <br />ajnbient <br />lab levels <br />ambient lab levels <br />Light Intensity footcandles) <br />4370 <br />' 50 -100 ft -c <br />50 -100 ft -c <br />Photo period <br />10 hr li ht/ 8 hr diork <br />16 hr light/ 8 hr dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume <br />30 nil- <br />300 mL <br />Renewal of Test Solutions <br />;every 24 hours <br />every 24 hours <br />Age of Test Organisms <br />less than 24 hours <br />less than 24 hours <br /># of neonates per test chamber <br />1 <br />10 <br /># replicate test chambers per conc. <br />10 <br />4 <br />Feeding regime <br />0.2 mL algae /YTC per day <br />0 15 mL A nauplii 2xdaily <br />Dilution Water <br />MHFSW <br />MHFSW <br />Test Duration <br />7 --8 days <br />7 -days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling Event 1 <br />Sampling Event 2 <br />Sampling Event 3 <br />MHFSW <br />H <br />7.88 <br />7.69 <br />7.87 <br />8.11 <br />Dissolved Oxygen m 1L) <br />7.30 <br />7.56 <br />7.61 <br />6.91 <br />Conductivity (pmhos /cm ) <br />3860 <br />4370 <br />4340 <br />314 <br />Ammonia (m /L) <br />0.03 <br />0.08 <br />0.06 <br />ND <br />Alkalinity (m /L ) <br />326.88 <br />313.66 <br />322.08 <br />57.69 <br />Hardness (m /L) <br />2140.93 <br />1 2470.77 <br />2514.75 <br />97.75 <br />Residual Chlorine (mg /L) <br />ND <br />ND <br />ND <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />IC25 <br />NOEC <br />LOEC <br />Ceriodaphnia dubia <br />Survival <br />>100% <br />100% <br />>100% <br />Ceriodaphnia dubia <br />Reproduction <br />r) 22.55% <br />12.5% <br />25% <br />Pimephales promelas <br />Survival <br />i >100% <br />100% <br />>1000 <br />Pimephales promelas <br />Growth <br />�i >100% <br />100% <br />>100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Average Number of Neonates <br />Control <br />10 <br />100% <br />35.6 <br />12.5% <br />10 <br />100% <br />34.1 <br />25% <br />10 <br />100% <br />24.9 <br />50% <br />10 <br />100% <br />22.0 <br />75% <br />1 10 <br />1 100% <br />14.6 <br />100% <br />1 10 <br />100% <br />4.4 <br />