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Aqua-Tox, Inc. <br />4880 Robb Street, Suite 8 <br />Wheat Ridge, CO 80033 <br />(303) 621-4880 wwvv,aqtia-tox com <br />ACUTE TOXICITY REPORT <br />SAMPLE ID: 2000-87 TEST RESULT LC60: >100% <br />CLIENT NAME: 20 Mile Coal CompariV 50% MORTALITY TEST- PASS <br />ATI LOT #: 031814-2 3181472 % SURVIVAL 100%: 100% <br />SAMPLE DATE 3/1712014 TIME: 11:30A ORGANISM: Da bnia magna <br />TEST START: 03-18-14 12:OOP ORGANISM AGE: <24 hours -old <br />TEST FINISH: 03-20-14 12:OOP <br />YPE OF TEST: Acute, LC,n, 48-hour, static, _renewal moderately hard fresh synthetic dilution water <br />Y <br />AMMONIA: <br />ND <br />HARDNESS: <br />94 44 mg/L <br />ALKALINITY' <br />55.74 mg/L <br />CHLORINE: <br />ND <br />DATE (HOURS) CONTROL 6 25 <br />3/18/2014 INITIAL <br />3/19/2014 24 <br />3/20/2014 48 <br />MORTALITY <br />% SURVIVAL <br />:)N WATER CONSTITUENT <br />D.O.: <br />7.35 mg]L <br />pH: <br />7.99 <br />CONDUCTIVITY- <br />310 microS <br />TEMPERATURE, <br />20.1 deg C <br />DILUTIONS (%) <br />125 25 5n 7 <br />20 <br />20 <br />20 <br />20 <br />_T___20 <br />20 <br />20 <br />j <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />100% <br />100% <br />1 100% _j <br />T 0 <br />100% <br />0 <br />100-% <br />0 <br />-100%-- <br />0 <br />100% <br />ILY CHEMISTRY - (MIN/MAX) DILUTIONS <br />CONTROL 625 12.5 25 50 75 100 <br />(.CINDUN:TJVITY ootcroS) 31 <br />DO (mqiL,__7 <br />pH 8 <br />TEMPERATURE (deg C 20 <br />0/347 <br />3491377 <br />377/399 <br />429/445 544/558 648/666 763/785 <br />4/7.6 <br />7.317.5 <br />7 3/7.5 <br />7.317.7 7.2/7.9 7,1/7,6 7 1/7.4 <br />0/8.1 <br />8.1/8.1 <br />_iO <br />8A/8.1 <br />8.1/8.1 8.0/8.1----8.0/8.2 7.9/8.2— <br />_ <br />� _/20 _ 7 <br />.1/20.i <br />20.1/20.7 <br />20.1/20.7 20.1/20.7 20.1/20.7 20.1/20 7 <br />RY - 2000-84 <br />AMMONIA: <br />0.01 mg /I_ <br />HARDNESS: <br />323.21 mg/L <br />ALKALINITY. <br />312.00 mg/L <br />CHLORINE: <br />ND <br />DOmmai• 7.08 <br />DOafter aeration NA <br />pH: 7.89 <br />CONDUCTIVITY: 763 micros <br />NOEL �FT LOEC CALCULATION METHOD METHOD REFERENCE <br />f SPEARMAN - KARBER I PA -821-R 02-012 October 2002 pg.78 <br />DITIONAL COMMENTS <br />Analysis <br />Conducted by: Reviewed bv,, <br />Date: f Date; <br />