2014-02-04_HYDROLOGY - C1982056 ‎(5)‎

• Permit No: C1982056
• IBM Index Class Name: Hydrology
• Doc Date: 2/4/2014
• Doc Name: 4th Quarter 2013 DMRS ‎(CO-0027154)‎
• From: Twentymile Coal Company
• To: WQCD
• Permit Index Doc Type: DMR’s
• Email Name: JDM
• Email Name: DIH
• Media Type: D
• Archive: No

Aqua -Tox, Inc.
J 4880 Robb Street, Suite 8
Wheat Ridge, CO 80033
(303) 621 -4880 www aqua- tox.com
P.
ACUTE TOXICITY REPORT
Daphnia magna
CLIENT NAME: Twent mile Coal Company SAMPLE I.D.: 101513 -1
50 % MORTALITY TEST: Pass TEST RESULT LC50: >100%
% SURVIVAL IN 100% DILUTION: 95%
SAMPLE DATE: 10 -14 -13 TIME: 111:05A CLIENT ID: 2000 -84
ORGANISM: Daphnia mom? AGE: < 24 hours old
TEST START: 10-15-1309:30A TEST FINISH: 10 -17 -13 09:30A
TYPE OF TEST: LC50, 48 hours, static, renewal, moderately hard fresh_svnthetic dilution water.
) FRESH SYNTHETIC DILUTION WATER CONSTITUENT
AMMONIA: ND _ D.O.: 6.77 mg /L
HARDNESS: 95.60 mg /L CHLORINE: ND
ALKALINITY: 55.65 mg /L TEMPERATURE: 20.1 deg C
pH: 8.08
SURVIVAL DATA DILUTIONS ( %)
DATE (HOURS) CONTROL_ 6.25 12.5 25 50 75 100
10/15/2013 INITIAL
10/16/2013 24
10/17/2013 48
MORTALITY:
% SURVIVAL_:
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
19
0
0
0
0
0
0
1
100%
100%
100%
100%
100%
100%
95%
Y CHEMISTRY - (MIN /MAX) DILUTIONS ( %,)
0 6.25 12.5 25 50 75 100
CONDUCTIVITY (micros;
D O (mg/L;
pH
TEMPERATURE (deg C'
298/343
540/624
686/775
998/1124
'1544/1771
2410/2460
3040/3130
6.8/7.0
6.8/7.()
6.8/71
6.8/7.3
6.8/7.8
6.8/8.1
6.8/8.4
7.8/8.1
7.9/82
8.1/8.3
8.1/8.4
8.3/8.5
8.4/8.8
8.3/8.9
20.1/20.6
20.1 /20.6
20.1 /20.6
20.1/20.6
20.1/20.6
20.1/20.6
20.1 /20.6
ERAL CHEMISTRY - SAMPLE
AMMONIA: 0.14 mg /L D.O.
HARDNESS: 641.01 mg /L CHLORINE
ALKALINITY: 477.46 mg /L TEMPERATURE
pH: 8.32 D.0 AFTER AERATION
ANOEC CALCULATION METHOD
100% SPEARMAN- KARBER EPA -E
ITIONAL COMMENTS
Analysis -.
r
Conducted by:
� f
Date: � - Z
8.35 mg /L
ND
20.1 deg C
NA
METHOD REFERENCE
21 -R -02 -012 October 2002 pg.78
Reviewed by:
Date; %f -
/-"/,7,/j 4