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Twentymile Coal Company - Site 62 Chronic Whole Effluent Toxicity Results <br />4th Quarter 2013 ATI Lot # 101513 -1abc <br />Summary of Laboratory Conditons & Bioassay Results <br />Parameter <br />Ceriodaphnia dubia <br />Pimephales promelas <br />Test Tvpe <br />7 -dav static renewal <br />7 -day_ static renewal <br />Temperature (deg C) <br />25 +/ - 1 deg C <br />25 +/ - 1 dea C <br />Light Quality <br />ambient lab levels <br />ambient lab levels <br />Light Intensity (footcandles) <br />50 -100 ft -c <br />50 -100 ft-c <br />Photooerlod <br />16 hr light/ 8 hr dark <br />16 hr light/ 8 hr dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume! <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />every 24 hours <br />every 24 hours <br />Age of Test Organisms <br />less than 24 hours <br />less than 24 hours <br /># of neonates per test chamber <br />1 <br />10 <br /># replicate test chambers e!r conc. <br />10 <br />4 <br />Feeding regime <br />0.2 mL algaeNTC per day <br />0 15 mL A nauplii 2xdaily <br />Dilution Water <br />MHFSW <br />MHFSW <br />Test Duration <br />7 -8 days <br />7 -days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling Event 1 <br />Sampling Event 2 <br />Sampling Event 3 <br />MHFSW <br />pH <br />7.92 <br />8.10 <br />8.09 <br />8.03 <br />Dissolved Oxygen (m /L) <br />7.35 <br />7.54 <br />8.31 <br />6.52 <br />Conductivity (pmhos /crn ) <br />3040 <br />3080 <br />3190 <br />290 <br />Ammonia (ma /L) <br />0.04 <br />0.01 <br />0.02 <br />ND <br />Alkalinitv (ma /L) <br />285.87 <br />288.68 <br />1 314.30 <br />55.65 <br />Hardness (mo /L) <br />1844.03 <br />1896.03 <br />1964.03 <br />95.60 <br />Residual Chlorine (ma /L) <br />ND <br />ND <br />ND <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />IC25 <br />NOEC <br />LOEC <br />Ceriodaphnia dubia <br />Survival <br />>100% <br />100% <br />>100% <br />Ceriodaphnia dubia <br />Reproduction <br />81.85% <br />75% <br />100% <br />Pimephales promelas <br />Survival <br />7 >100% <br />100% <br />>100% <br />Pimephales promelas <br />Growth <br />>100% <br />1 100% <br />JLt >100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Mean Average Number of Neonates <br />v <br />Control <br />10 <br />90% <br />26.8 <br />12.5% <br />10 <br />100% <br />29.6 <br />25% <br />10 <br />100% <br />28.4 <br />50% <br />10 <br />100% <br />28.4 <br />75% <br />10 <br />11 100% <br />23.5 <br />100% <br />10 <br />1 100% <br />15.2 <br />