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Twentymile Coal Company - Site 62 Chronic Whole Effluent Toxicity Results <br />3rd Quarter 2013 ATI Lot # 081313 -1 abc <br />Summary of Laboratory Conditons & Bioassay Results <br />Parameter <br />Ceriodaphnia dubia <br />Pimephales promelas <br />Test Tvpe <br />7 -day static renewal <br />7 -day static renewal <br />Temperature (dea C) <br />25 +/ - 1 deg C <br />25 +/-1 dea C <br />Light QualityV <br />ambient lab levels <br />ambient lab levels <br />Light Intensity (footcandles) <br />50 -100 ft -c <br />50 -100 ft -c <br />Photoperiod <br />16 hr light/ 8 hr dark <br />16 hr light/ 8 hr dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />every 24 hours <br />every 24 hours <br />Age of Test Organisms <br />less than 24 hours <br />less than 24 hours <br /># of neonates per test chamber <br />.1 <br />10 <br /># reolicate test chambers per conc. <br />10 <br />4 <br />Feeding regime <br />0 -2 mr algae /YTC per day <br />0 15 mL A nauplu 2Xdaily <br />_ Dilution Water <br />MHFSW <br />MHFSW <br />Test Duration <br />m 7 -8 days <br />7 -days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling Event 1 <br />Sampling Event 2 <br />Sampling Event 3 <br />MHFSW <br />pH <br />7.99 <br />7.84 <br />787 <br />8.05 <br />_ Dissolved Oxygen (mg/L) <br />Reproduction <br />6.38 �m^ _ <br />6.40 <br />_ <br />6.81 <br />Conductivity (umhos /cm) <br />4w_ <br />3030 <br />3020 <br />2960 <br />328 <br />Ammonia (mg /L) _ <br />ND <br />0,01 <br />0.01 <br />ND <br />Alkalinity (mg /L) <br />247.97 <br />231.94 <br />189.88 <br />52.88 <br />Hardness (mg /L) <br />2405.19 <br />2163.67 <br />2319.36 <br />84.63 <br />- -- Residual Chlorine (mg /L) <br />ND <br />ND <br />ND <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />IC25 <br />NOEC <br />LOEC <br />Ceriodaphnia dubia <br />Survival <br />tg. >100% <br />100% <br />>100% <br />Ceriodaphnia dubia <br />Reproduction <br />r . 69.40% <br />50% <br />75% <br />Pimephales promelas <br />Survival <br />j >100% <br />100% <br />>100% <br />Pimephales promelas <br />Growth <br />>100% <br />100% <br />t. `, >100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Mean Average Number of Neonates <br />Control <br />10 <br />100% <br />35.2 <br />12.5% <br />10 <br />100% <br />34.9 <br />25% <br />10 <br />100% <br />37.1 <br />50% <br />10 _ <br />90% <br />31.3 <br />75% <br />10 <br />100% <br />25.5 <br />100% <br />10 <br />100% <br />21.0 <br />