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<br />if possible, and compared with historic N,. This metric may be evaluated as a means to <br />determine critical population size for taking fish into captivity into a refuge or for captive <br />propagation. <br /> <br />Alternatives for the populations of Humpback Chub in the Upper Colorado River Basin (in <br />priority) <br /> <br />3. Continue to address threats to the species and translocate young-of-the-year (yoy; when <br />available) from nearest populations to river reaches where threats have been reduced or <br />minimized. For example, the Yampa Canyon population might be supplemented with <br />Desolation/Gray yoy once the control of nonnatives is affected in Yampa Canyon. <br />Suggest the movement of no less than 100 yoy per year for at least 5 years. <br /> <br />4. Move yoy to a refuge, i.e., a hatchery such as Ouray or Grand Valley, and expect to add <br />yoyon an annual basis to minimize any genetic changes caused by genetic dri ft or <br />adaptations to hatchery conditions. In addition, funding will be required to provide <br />operation and maintenance of the fish held at the refuge. Identifying refuge, getting <br />permits lined up for signature, and other associated logistics of getting fish out of the wild <br />could be in place in case this is deemed necessary. <br /> <br />5. If a precipitous decline in all populations occurs and is validated', then breeding matrices <br />(e.g.. 25x25 paired matings) should be developed that utilize many individuals from the <br />five diverse populations throughout the Upper Colorado River Basin, with the goal to <br />maintain genetic variation of the original wild populations. <br /> <br />6. Augmentation of the populations should not occur until the threats have been removed or <br />minimized to a level that reintroduced individuals can survive, mature, reproduce, and <br />their progeny do the same. <br /> <br />Alternatives for the Grand Canyon Population of Humpback Chub (ill priority) <br /> <br />3. Continue to address threats to the species and translocate yay (when available), that <br />would presumably be lost to the mainstem Colorado River, into tributaries where threats <br />have been reduced or minimized. This strategy of translocation has been preliminarily <br />successful in expanding the population in the Little Colorado River in an upstream <br />direction, past a natural fish barrier, Chute Falls (personal communication. Pam <br />Sponholtz, U.S. Fish and Wildlife Service). Suggest the movement of no less than 100 <br />yoy per year for at least 5 years. Continue to explore other tributaries that may be suitable <br />for translocations. <br /> <br />4. Move yoy to a refuge, i.e., a hatchery such as Willow Beach or Wahweap, and expect to <br />add yoy on an annual basis to minimize any genetic changes caused by genetic drift or <br />adaptations to hatchery conditions. In addition, funding will be required to provide <br />operation and maintenance of fish held at the refuge. Identifying refuge, getting permits <br /> <br />DRAFT HBC Genetics Management Plan - 6 <br /> <br />n(lhl;? <br />