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c' ,-., ~... I .. 0-:> o channels are defined as ehannels having less than 25% of the volume of flow at the time of sampling and are at least 300 m in length. Inflow at the top of a channel is not necessary for it to be classified as a secondary channel. If any portion of a secondary channel (except debouchrnent) is within a DM, the secondary channel will be sampled. Young-of-year/small-bodied fish monitoring will occur during mid-September to mid-October each year. All secondary channels in each DM will be sampled. Primary channel shoreline habitats and off-shore riffles also will be sampled in 3-mile increments. Primary channel samples may be obtained throughout the DM. In addition to structured primary channel sampling, all backwaters and embayments (>25 m2) associated with the primary channel within the DM will be sampled. Sampling within secondary channels will be a sufficient distance from the inflow to and outflow from the secondary channel to minimize primary channel faunal and physiochemical influences. All mesohabitats (i.e., backwater, pool, shoal, run, and rifile) present within the secondary channel will be sampled in approximate proportion to their availability within the secondary channel. Each mesohabitat will be sample separately with 3,2 x 1.6 or 3.8 x 1.6 (4 mm mesh) double-weighted drag seines. A 12 v backpack eleetrofisher may be used in eombination with a block seine to sample riffle habitats. Sufficient effort will be made to ensure that a representative sample of specimens from each sampled mesohabitat is obtained. The number of seine hauls, total area (m2) seined, and types of meso habitats sampled will be recorded on standard field forms (Figure ). Specimens collected in each mesohabitat will be inspected to determine if any rare fishes (Colorado pikeminnow, roundtail chub, and razorback sucker) are present in the seine, If a rare fish is captured, it will be identified, total length C:!: 1 mm) and weight C:t 1 g) determined, and released. Any rare fish> 150 mm TL will be scanned to determine presence of a PIT tag. If none is present, the speeimens will be implanted with a PIT tag. All pertinent data (i.e., total and standard lengths, weight, PIT tag code, rnesohabitat, water depth, substrate, and cover) on rare fish captured will be recorded. All sub-adult and adult large-bodied native fish (i.e., flannelmouth and bluehead suckers) will be weighed, measured, and released. All other specimens will be preserved in 10% formalin and returned to the laboratory for identification, enumeration, and measurement (total length). Field colleetion number, habitat number, and river mile will be recorded on a waterproof label and placed in each specimen container. Location of site (latitude and longitude) will be determined \vith a GPS unit. Identification of all retained rare fishes will be confirmed by personnel of the Museum of Southwestern Biology. Preserved specimens will be accessioned to the Museum of Southwestern Biology. Within each DM, shoreline and "seineable" off-shore habitats of the primary channel will be sampled. Within each DM, all mesohabitats types (i.e., backwater/embayment, pool, shoal, run, and riffle) present will be sampled. Each mesohabitat present will be sampled in approximate proportion to its availability within the site. Specimens will be collected with a double-weighted drag seine (3.8 x 1.6 or 3.2 x 1.6 m, 4 mm mesh). A 12 v backpack electro fisher will be used in combination with a block seine (4 mm mesh) to obtain specimens from riffles. Samples may be obtained at several locations within each DM to ensure that all mesohabitats within the mile are sampled. Location (latitude and longitude) of each meso habitat sampled will be determined with a 6 Drall Long TCnTI Monitoring Plan - SJRlP Biology Comlluncc