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<br />.oj <br /> <br />C') <br />.c:') <br />'"., <br />,:;". <br />~ <br />C,.>1 <br /> <br />significant interaction between treatment and day (F=5 .12, P<O.O l). The interaction of treatment <br /> <br />and day indicated that selenium accumulation in muscle tissue of females over time was not the <br /> <br />same across treatments. Multiple mean comparisons showed that muscle plug selenium <br /> <br />concentrations in fish exposed to treatment 2 were similar to those of control fish (treatment I) at <br /> <br />all sample periods. At day 58 of exposure, selenium concentrations in muscle plugs of females <br /> <br />exposed to the high selenium-fortified diet with or without selenium-spiked water were <br /> <br />significantly greater than those of the controls. From day 90 to the end of the study, muscle plug <br /> <br />selenium concentrations in females exposed to either selenium-fortified diet with or without <br /> <br />selenium-spiked water were higher than those in control fish, except for fish in treatment 3 at day <br /> <br />245. Ranges in female muscle plug selenium concentrations after 153 days of exposure were 1.1 <br /> <br />to 1.3 ,Lg/g for treatment I, 1.2 to 1.3 flg/g for treatment 2, 1.5 to 2.1 i-1g/g for treatment 3, 2.0 to <br /> <br />2.3 flg/g for treatment 4,2.5 to 5.2 i-1g/g for treatment 5, and 2.0 to 3.0 pglg for treatment 6. <br /> <br />Selenium concentrations in muscle plugs of the one male fish from treatment 2 ranged from only <br /> <br />1.2 to IA flg/g across all sample days. <br /> <br />For fish fed the low selenium-fortified diets, there were no differences in muscle plug <br /> <br />selenium concentrations between the control water and selenium-spiked water treatments at any <br /> <br />of the sample periods (Table II). for the high selenium dietary treatments, fish exposed to <br /> <br />control water had higher muscle plug selenium concentrations than those exposed to 7.94 i-1g/L <br /> <br />selenium in the water from day 90 to the end of the study. These results indicated that <br /> <br />waterborne selenium exposures of about 5-8 pg/L for 153 days did not significantly influence <br /> <br />selenium accumulation in epaxial muscle at dietary selenium concentrations of 2.18-11.83 ilg/g. <br /> <br />Additional analysis using time-weighted average selenium exposure concentrations for <br /> <br />".: each exposure period and tank revealed that selenium concentrations in muscle plugs at days 58, <br /> <br />:..i <br /> <br />1.0. <br /> <br />43 <br />