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42 With protective prOV1Slons included in the reasonable and prudent alternative contained herein, the Service does not anticipate that construction and operation of the proposed Project will result in any incidental take of Colorado squawfish or razorback sucker. The Service anticipates that a small number (not to exceed 3 adults Colorado squawfish or 3 adult razorback sucker) of endangered fish could be taken as a result of the research program which is part of the reasonable and prudent alternative to preclude jeopardy. Incidental take would be associated with activities, such as capture, holding, or transporting fish, required by the research program, but would be covered by threatened and endangered species collection permits and would require immediate notification and cessation of any activity resulting in a take until further Service review. The following reasonable and prudent measure is necessary and appropriate to minimize take and is required for all research funded or implemented by Reclamation. A permit which will include measures to reduce take will be obtained in accordance with 50 CFR 17.22 and 32 from the Fish and Wildlife Service. In order to be exempt from the prohibitions of section 9 of the Endangered Species Act, the applicant must comply with the following terms and conditions which implement the reasonable and prudent measure described above. 1. Every effort will be made to prevent mortality of young-of-the-year fishes. Only those fish that cannot be accurately identified in the field may be sacrificed. 2. The following electrofishing procedures will be implemented. a. Electrofishing equipment should be calibrated each year under controlled laboratory conditions. Wave forms should be measured to ensure that spiked wave forms are not being produced and that no reversal of polarity is encountered. b. Only persons with field or formal training in electrofishing methods and one or more field seasons of experience are authorized to use this method. c. Electrofishing should use direct current (D.C.) only. When pulsed D.C. current is used, rectangular wave forms at pulse frequencies of 40 pulses per second or less should be used. d. Electrofishing should be restricted to waters in which conductivity measures less than 1,000 micro mhos per cm. e. All tissue collection shall follow the protocol outlined in the report titled "Razorback Sucker Genetic Survey--Tissue Sampling Protocol."