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<br />25 <br /> <br />through single exposures to microgram quantities. The PAHs act at both the <br />site of application and at organs distant to the site of absorption; their <br />effects have been demonstrated in nearly every tissue and species tested, <br />regardless of the route of administration (Lee and Grant 1981). The evidence <br />implicating PAHs as inducers of cancerous and precancerous lesions is <br />overwhelming, and this class of substances is probably a major contributor to <br />the recent increase in cancer rates reported for industrialized nations (Cooke <br />and Dennis 1984). <br /> <br />Ongoing Service analyses of PAH contamination of aquatic biota of the San Juan <br />River and hepato-histological examinations of fish in the river have raised <br />concerns regarding the exposure of these organisms to contaminants introduced <br />into the basin through the intensive development of energy resources in the <br />area. Analyses of bile samples taken from fish in the San Juan River indicate <br />that these organisms are being exposed to high levels of three PAH compounds, <br />and strongly suggest that the aquatic environment of the river is heavily <br />impacted by'PAHs. <br /> <br />The primary PAH compounds of concern in the San Juan River are naphthalene, <br />phenanthrene, and benzo(a)pyrene. The lower molecular weight unsubstituted <br />PAH compounds, such as naphthalene and phenanthrene, have significant acute <br />toxicity to some organisms (Krahn et al. 1984, 1986: Zitko 1975). Higher <br />molecular weight PAHs, including benzo(a)pyrene, have been found to cause <br />mutations and cancer in aquatic and terrestrial organisms (Eisler 1987). <br />Background concentrations of these compounds are <10,000 nanograms per gram <br />(ng/g) for naphthalene, <3,000 ng/g for phenanthrene, and <100 ng/g for <br />benzo(a)pyrene (National Marine Fish. Servo 1989). Flannelmouth suckers <br />(Catostomus latiDinnis) (n=36) analyzed from the San Juan River had mean <br />concentrations of 97,110 ng/g wet weight naphthalene, 15,767 ng/g <br />phenanthrene, and 256 ng/g benzo(a)pyrene in bile samples. From these data, <br />it is apparent the flannelmouth suckers in the San Juan River are exposed to <br />and accumulating PAH compounds in bile. Utilizing the flannelmouth suckers as <br />indicators of similar exposures experienced by the Colorado squawfish and <br />razorback sucker, these levels are of significant concern. <br /> <br />Physical Habitat <br /> <br />The impacts to physical habitat involve the loss of the quantity and quality <br />of water in critical habitat and the change in flow regime. The quantity and <br />timing of flows influence how various habitats are formed and maintained. <br />Water depletions reduce the ability of the river to create and maintain these <br />habitats; degradation of water quality lessens the ability of endangered <br />species to survive in these habitats. <br /> <br />Osmundson and Kaeding (1991) reported observations on the Colorado River <br />(15-mile reach) during the drought years of 1988 -1990, that backwaters were <br />filling in with silt and sand because spring flows were not sufficient to <br />flush out the fine sediment. Also they reported that tamarisk colonized sand <br />and cobble bars, stabilizing the river banks. On the San Juan River, lack of <br />flooding since Navajo Dam was completed and introduction of exotic riparian <br />vegetation (tamarisk and Russian olive) has armored the channel banks <br />