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<br />8 <br /> <br />Chronological Order of Data Analysis <br /> <br />The contract stated that each methodology should be evaluated <br />individually, step by step and with as little bias as possible <br />occurring between methods. The primary investigator and the field <br />crew under his supervision collected all of the field data, thereby <br />at least maintaining a constant source of error and hopefully as <br />small an error as possible. The field data for the R-2 Cross analysis <br />process was turned over to the Ecological Services Section of the DQW <br />for computer analysis and determination of minimum flows by the Sing~ <br />Transect R-2 Cross Method. This was done without any assistance or <br />input from the primary investigator. <br /> <br />After the single transect R-2 Cross m~n~mum flow recommendations <br />were made by Ecological Services, all R-2 Cross data were then turned <br />over to the primary investigator for the multiple transect R-2 Cross <br />analysis and minimum flow determination process, without the primary <br />investigator's knowledge of what the single transect R-2 Cross minimum <br />flow determination was. <br /> <br />Finally, these sets of data (single and multiple transect cross <br />section R-2 Cross analysis) were set aside and the IFG4 analysis was <br />completed using average velocity and average depth as the two criteria <br />for making minimum flow recommendations. <br /> <br />The Tennant or Montana Method was the fourth method used and is <br />a synthetic analysis based on mathematical manipulation of U.S.G.S. <br />gaging data as described by Tennant (1975). Thirtv percent of the <br />average discharge, which Tennant classifies as an excelleQt flow for <br />the needs of fish and aquatic invertebrates. was the level used in <br />this study for comparative purposes between methods. <br /> <br />The last method of analysis was the ,IFG3 or "weighted usable, <br />.area" model (Bovee and Cochnauer, 1977) which interfaces with both <br />the IFG4 Incremental Method and the Wetted Surface Profile (WSP) or <br />IFG2 Method. In this study, the IFG3 was interfaced with the IFG4 <br />Method, but not in the traditional sense for setting minimum flow <br />recommendations. Rather, weighted usable area for all life stages <br />was analyzed to determine if the model predictions were reflected in <br />the actual species composition of the fish population observed through <br />electroshocking studies. <br /> <br />Population Estimation Methodologies <br /> <br />Trout population density and biomass estimates were completed <br />using the standard Petersen Mark and Recapture method with two electro- <br />shocking runs through the stream. In most instances 24 hours of time <br />elapsed between the first pass for marking and the second (recapture) <br />pass. However, on the small streams with less than 10 cfs discharge <br />the second pass was made the same day. <br />