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<br />575 <br /> <br />The biological measures in the test with adults will include water residues of selenium (samples <br />collected at IS-day intervals), dietary residues of selenium (samples collected from each bag of diet), <br />tissue residues of selenium (muscle plug samples, includes depuration), survival (recorded daily -- no <br />adults are expected to die during the study), growth (length and weight measured), behavior <br />(recorded weekly), number of eggs spawned, and number of eggs hatched. <br /> <br />The biological measures for the 30-day larvae study will include water residues of selenium (samples <br />collected at IS-day intervals), dietary residues of selenium (samples collected from each bag of diet), <br />tissue residues of selenium (samples collected at the end of the 30-day study), fish survival (recorded <br />daily), growth (length and weight measured at the end of the study), behavior (recorded weekly), and <br />swimming perfonnance (tested after 30 days of exposure). <br /> <br />The biological endpoints from the adult and larval studies will be compared to each other to derive <br />inter-relations of various measures (i.e., tissue residue vs. effects on growth) and compared to <br />environmental data from the synoptic survey conducted by others to derive a hazard assessment of <br />the potential of the waterborne and dietary selenium to adversely affect threatened or endangered fish <br />in the San Juan River. <br /> <br />Budget: <br /> <br />(Some funding obligated in FY96 ($25,000) has been incorporated into the FY97 budget -- the <br />adjusted figures are given below.) <br />Long-tenn reproduction test with adult Colorado squawfish. <br />(cost share between SJRRIP and NIWQP1) <br />Personnel <br />Travel/Per Diem <br />Equipment and Supplies <br />Selenium diet <br />Neutron activation analysis (adult muscle plug) <br />Neutron activation analysis (larval fish) <br />Chemical analysis <br />Tanks, remodel diluter, delivery lines, etc. <br /> <br />SJRRIP <br />$21,600 <br />2,000 <br />4,000 <br />7,000 <br />14,400 <br />6,000 <br />16,000 <br />o <br /> <br />Subtotal <br />NBS Administrative Overhead - 19% <br />Proposed Budget FY97 <br /> <br />71,000 <br />13.500 <br />$84,500 <br /> <br />lSJRRIP: San Juan River Recovery Implementation Program. <br />NIWQP: National Irrigation Water Quality Program. <br /> <br />35 <br /> <br />NIWOP <br />10,800 <br />1,000 <br />2,000 <br />3,500 <br />7,200 <br />3,000 <br />8,000 <br />6.000 <br /> <br />41,500 <br />7.900 <br />49,400 <br />