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<br />. . . <br /> <br />v. Study Mathods/Approach: <br /> <br />Passive drift-net sampling is an effective and proven method for capturing <br />Colorado squawfish larvae. In properly conducted, results can provide & <br />reascmal:lle estilllace of annual reproductive output frOlll spawning agrees <br />without over-sampling larvae. <br /> <br />Drift-net sampling in the Yampa and Green rivers, as part of FGFRI, was <br />started in 199,0 and will be conducted again in 1991. In 1991, two drift- <br />net sta.t:ionswere established, i. e., lower Yampa River at Rcho Park and <br />Green River just belew the Split Mountain boat ramp. At each station, <br />three flow-metered plankton nets were set near the shore, daily at dawn <br />for 1-2 h, frOlll end of June through early August. The objectives of this <br />sampling were to doCUlllent timing and duration of spawning by Colorado <br />squawfish in the lower Yampa River, determine peaks in abundance of <br />transported Colorado squawfish larvae transported frOlll the lower Yampa <br />River spawning area into nursery habitats in the Jensen-Ouray area; <br />nursery-habitat sampling conducted by Utah DWR (Core-Research study) in <br />fall 1990 was, intended to Subsequently determine abundance of young <br />Colorado squawfish in backwater habitats. Results of the 1990 drift-net <br />sampling were provided in an annual progress report sul:lmi tted to the 0. S . <br />Bureau of Reclamation. After discuseions with the cooperative FGFRI team <br />in December 1990, the drift-net sampling scheme used in 1990 was changed <br />in favor of the design proposed here (this new study design will be <br />employed in 1991) . <br /> <br />Reproductive output will be measured by daily captures of drifting <br />Colorado squawfish larvae at two sites, one at mouth of the Yampa River <br />and one below Grey and Desolation canyons in the lower Green River. Both <br />of these sites are below known spawning grounda, and data frOlll sampling at <br />these sites will provide estimates of relative abundance of Colorado <br />squawfish larvae being transported frOlll spawning areas and into potential <br />nursery habitats. <br /> <br />Colorado squawfish in the Yampa River spawn on the descending limb of the <br />hycirograph, often asso<:iated with abrupt increases (flow spikes) in base <br />flew (Nesler et al. 1988; Tyus and Karp 1989). Sampling for Colorado <br />squawfish larvae will be initiated based on those data ,and stream-flow <br />conditions prior to sampling (probably start in mid-late June). Duration <br />of the sampling period will depend on number of larvae collected in late- <br />season samples, past data, and stream-flow conditions probably end early- <br />mid August) . <br /> <br />Colorado squawfish larvae are most abundant in drift-net samples at dawn <br />and nearshore and midetream nets capture roughly equivalent numbers of <br />fiSh/unit volume of water sampled (Haynes et al. 198.; Nesler 1986). This <br />was supported by results of out nearshore sampling during SUlllller 1990; <br />catch rates of three nearshore drift nets were similar (one-way ANOVA) . <br />Results of the 1990 sampling showed that about 60t of drifting Colorado <br />period on only four sampling days; .. determined that daily sampling over <br />a 6-S-week period was necessary to detect and measure peaks in larval <br />abundance and brack6t the period of larval transport. <br /> <br />At each station, three .-m-long conical plankton nets (560 micron mesh) <br />will be set deily at dawn. Nets will be attached to rectangular steel <br />frames (0.15 m') and staked into the stream substrate adjacent to the shore <br />in water 0.5-1.0 m deep. A rttDlClVahle collection bucket for trapping <br />filtered lIIaterial and fishes will be attached to the cod end of each net. <br />Flow meters for measuring velocity will be suspended inside the mouth of <br />each net, and net sets will be timed to determine volume of water sampled. <br />Duration of each set will be 1-2 h depending on debris load. Samples will <br />be fixed and preserved in 95-100t ethanol (for subsequent otolith-ageing <br /> <br />Flaming Gorge Studies <br /> <br />A. <br />