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<br />5 <br /> <br />Relative density of nonnative fishes should nO! be a selection criteria, <br />It will be a response variable. <br />Comments on Evaluation and Refinement of Design Criteria (page 11). <br /> <br />The list of response variables is very long and will ultimately <br />complicate analyses of the data. I would select a small number of response <br />variables (i.e., CPUE of native fish, CPUE of nonnative fish I as primary, and <br />additional data as defined by your long list can simply be supportive <br />information around the primary hypothesis testing. In' this manner you will <br />maximize your chance to conclude something concrete from your research <br />and it will allow you to develop an implementation plan based on sound <br />significant differences or none as the case may be. Many response <br />variables will dilute the final conclusions so much that you may never finish <br />the research and get on to management. <br /> <br />Comments on Evaluation Design/Methods (pages 11 and 12). <br /> <br />\ will re'lterate the importance of a standard survey method. One <br />sampling protocol should be adopted to provide the data to test your null <br />hypotheses. Other sampling can be done as man-power dictates, but unless <br />one standard method is established that can be replicated in each test site <br />type you will have insufficient statistical power to reject or accept the null <br />hypothesis with any precision. I think that the standard survey shculd be <br />designed to sample the test site as a un'it, not individual habitats (j.e., <br />depressions or terraces) within a test site. The amount Of specific habitats <br />in test sites should be quantified and shauld constitute diffe'ences among <br />test sites to be tested by the means differences testing statis~ic. A levee <br />removal site primarliy composed of terrace habitat should not be used as a <br />replicate for a levee removal site primarliy composed of depressions; <br />however, if you make an effort to restore flood function (i.e.. scouring <br />flowsl, after a year or so the river will re-work the site to its own design and <br />then your testing will have to be re-thought. <br /> <br />larval fish sampling must not be used as a response variable unless <br />these data can be quantified and qualified. Do larval light traps collect all <br />species in a random fashion in the proportion they exist in the population? <br /> <br />To the extent possible, a selection of gear types and fishing effort <br />should be adopted that will provide equalized effort, catch per unit effort <br />between test sites for all target species. In other words, it is not acceptable <br />to use trammel nets in manipulated sites and electro fishing in levee removal <br />sites to provide the response variable for means differences testing. <br /> <br />Macroinvertebrates can be a good indicator of differences between <br />test sites. You should adopt a standard protocol such as a series of <br />