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<br />. <br /> <br />o <br />o <br /><:) <br />..:::.. <br />C', <br />UI <br /> <br />- sever ends of gonads from vent, suture closed and tie and/or suture <br />loose ends together (loop) <br />- recovery <br />C. Mortality <br />freeze specimen <br />- use replacement fish from extra <br />- perform surgery as above <br /> <br />IV. Maintenance/Holding <br /> <br />A. Maintain current <br />- pump replacement/maintenance <br /> <br />B. Feeding <br />- per determined rate of ____ kg trout/kg body weight of CSF <br /> <br />c. Raceway maintenance <br />- appropriate cleaning <br />- screen maintenance <br /> <br />D. Water Quality <br />- temperature regulation where appropriate (will depend upon ambient <br />temperature effects on raceway temperatures, attempts to assimilate <br />wild temperature regime) <br />water replacement to make up for evaporative/other losses <br />thermograph placement for duration of holding <br />- monthly readings of D.O., Ph, conductivity, nutrients (N03) <br /> <br />V. Post surgery examination. data acquisition <br /> <br />. <br /> <br />A. Timing Late May/Early June <br />- based upon hatchery experiences but will be somewhat dependent upon <br />raceway temperatures <br /> <br />B. Examination of experimental fish <br />anesthetize, perform same Burgery as above to expose gonad ends <br />examine gonads for visibility (mature/maturing ovaries and tests) <br />examine former, gonadal attachment sites near vent <br />external appearance, other comments <br /> <br />C. Recovery of control fish <br />recover and place back into raceway <br />~ maintain until use or nonuse decided <br /> <br />D. Examination of control fish <br />record individual fish TL, SL, WT <br />- external appearance, other comments <br /> <br />VI. Reporting <br /> <br />A. Data analysis, writeup, reporting <br />- by August 3~, ~99~ <br /> <br />B. Recommendations <br />- Will it work if we need hatchery fish? <br /> <br />VII. Experimental and Control Fish Disposition <br /> <br />A. Maintenance until October ~992 <br />results of collection efforts for wild fish <br />- decision for use/nonuse <br /> <br />. <br /> <br />A~6 <br />