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<br />( <br /> <br />( <br /> <br />L <br /> <br />a <br />o <br />(--") <br />t:.~ <br />c.o <br />;1:::0 <br /> <br />e.) Sample storage <br /> <br />Place vials with tissues, sequentially in vial racks, in ice chest, <br />on dry ice as soon as possible. The main idea is to freeze them on <br />dry ice and move them into an ultracold freezer as soon as <br />possible. Once frozen, avoid thawing of tissues. <br /> <br />The attached protocol "PROCEDURE FOR COLLECTING TISSUES" is for <br />collecting gametes to be used in mitochondrial DNA analysis. <br /> <br />Included therein is a protocol for taking tissues from dead fish. <br />If fish is sent whole, put PIT-tag in dad fish, place label with <br />pertinent data in mouth of dead fish (including PIT-tag number), <br />wrap whole in aluminum foil, label, and freeze in dry ice as <br />quickly as possible. <br /> <br />The Fish and wildlife Service will be responsible for providing vials, <br />markers, notebooks, punches, scissors, forceps, etc. needed for tissue <br />collections. <br /> <br />AS <br />