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<br />( 0 <br /> 0 <br /> 0 1.) <br /> Co) 2. ) <br /> to <br /> l\:l 3. ) <br /> 4. ) <br /> 5. ) <br /> 6. ) <br /> 7. ) <br /> 8. ) <br /> <br />Protocol for Surgically Implanting Radio-transmitters <br /> <br />Fieh anesthetized with MS-222. <br /> <br />Gills bathed with anesthetic while performing surgery. <br />Fish covered with damp towels. <br /> <br />Transmicter coated with bees wax. <br /> <br />Transmitter and surgical tools sterilized with alcohol. <br /> <br />Needle threaded with approx. 50 an of suture material (ETHICON <br />MONOFILAMENT) <br /> <br />Incision area on fish sterilized with surgical scrub (Betadine). <br /> <br />Incision made laterally, immediately anterior and slightly dorsal <br />to insertion of right pelvic fin. About 1 t 1.5 inches in length <br />and just deep enough t severe the peritoneal lining. <br /> <br />9.) Remove tag from alcohol and insert into peritoneal cavity with <br />antenna pointing posteriorly. <br /> <br />10.) Incision closed with 5 to 8 individual sutures, tied with surgeons <br />knot. <br /> <br />( <br /> <br />11.) Entire procedure should be completed in 5 to 7 minutes. <br />12.) Wound area covered with Betadine. <br /> <br />13.) Fish placed into recovery tank until fully recovered from <br />anesthetic. <br /> <br />14. ) <br /> <br />15. ) <br /> <br />l. <br /> <br />Frequency of transmitter checked bsfore and after implantation into <br />fish. <br /> <br />Fish released back to river. <br /> <br />A3 <br />