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<br />hybridization. <br /> <br />V. Study area (river miles, if appropriate): This study will be conducted <br />at Wahweap State Fish Hatchery or another state facility. <br /> <br />VI. Study Methods/Approach: <br /> <br />PHASE I: F~ VIABILITY <br /> <br />We propose producing 49 F, paired crosses (Table 1). Seventeen paired <br />matings will produce pure Gila offspring. Thirty-two paired matings, will <br />produce hybrids between the Gila species. All matings will be held in <br />separate tanks or marked as individual family lots. <br /> <br />Table 1. All possible crosses (N=49) using replicates to estimate among-male <br />and among-female variance (B= Bony tail, H= Humpback, R= Rountail). <br /> <br />Male <br /> <br />F <br /> <br /> Bl B2 B3 HI H2 Rl R2 <br />Bl BlxBl B2xBl B3xBl HlxBl H2xBl RlxBl R2xBl <br />B2 BlxB2 B2xB2 B3xB2 HlxB2 H2xB2 RlxB2 R2xB2 <br />B3 BlxB3 B2xB3 B3xB3 HlxB3 H2xB3 RlxB3 R2xB3 <br />HI BlxHl B2xHl B3xHl HlxHl H2xHl RlxHl R2xHl <br />H2 BlxH2 B2xH2 B3xH2 HlxH2 H2xH2 RlxH2 R2xH2 <br /> .' <br />Rl BlxRl B2xRl B3xRl HlxRl H2xRl RlxRl R2xRl <br />R2 BlxR2 B2xR2 B3xR2 HlxR2 H2xR2 RlxR2 R2xR2 <br /> <br />e <br /> <br />m <br /> <br />a <br /> <br />1 <br /> <br />e <br /> <br />Transport adults to facility <br /> <br />During the fall-spring of the first study year, adult Gila will be obtained <br />from hatchery facilities or collected. We foresee using Gila cypha from <br />Horsethief or the wild, adult Gila elegaDs from Dexter, and collecting adult <br />Gila robusta from opper Basin rivers. We have the opportunity to collect wild <br />adult humpback chub from Westwater Canyon at the same time roundtail chub are <br />collected if the Program so desires. The humpback.chub could be held at <br />Wahweap if pond space is limiting at other facilities. . <br /> <br />Paired matings <br /> <br />Adults from each Gila spp. will be used for the crosses in the spring/early <br />summer of the first and second study years. The laboratory facilities at UDWR <br />Wahweap State Fish Hatchery will be used for all possible crosses between <br />Giia. Crosses within pure species will serve as controls (Table 1). <br />Approximately 500-1000 eggs from each female will be fertilized (a single male <br />will be used for each female). These eggs will be placed in hatching jars and <br />subsequently aquariums. <br /> <br />3 <br />