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<br />002779 <br /> <br />to the field to conduct sampling. Eleven adult razorback sucker were captured, PIT -tagged and <br />transferred to refugia at the Ouray EFCES in the spring of 1990. Milt and eggs were taken from <br />these fish and sent to ASU for mtDNA analysis. Four fish from the Upper Colorado River mainstem <br />were captured, PIT -tagged and transported to refugia at Dexter National Fish Hatchery, for a total <br />of seven adults at that facility. No tissues of any type were taken from these fish. Milt and eggs were <br />taken from Green RiverfYampa razorback sucker and sent to ASU. <br /> <br />By early 1991, non-lethal tissue sampling techniques were developed and employed with considerable <br />success. Field trials were conducted wherein fin and muscle tissues were taken from experimental <br />fish for allozyme analysis. The experimental fish were observed under controlled conditions at Ouray <br />EFCES for procedural effects after which tissues were analyzed in the laboratory for their use in <br />genetic analysis. With regards to Upper Basin razorback sucker genetic sampling, the following <br />information was gathered (U.S. Fish and Wildlife Service 199Ib). <br /> <br />Upper Colorado River mains/em, Grand Valley Area - Sampling sites were identified and <br />sampling times were determined by U.S. Fish and Wildlife Service (USFWS) and Colorado <br />Division of Wildlife (CDOW) staffs in Grand Junction, Colorado. In addition, 2 apparent <br />hybrids from Etter Pond, near Debuque, Colorado, and from Highline Reservior were captured <br />and frozen whole. Muscle and fin tissues from 5 additional apparent hybrids were sent to <br />researchers at AS U. <br /> <br />Upper Green RiverlYampa - Sampling sites were identified and sampling times (May through <br />June) were determined by Colorado River Fish Project staff in Vernal, Utah. <br />Green River razorback bar: (includes Escalante Ranch) Muscle and fin tissue samples were <br />taken from 30 razorback sucker, 30 flannel mouth sucker, 30 bluehead sucker, and 3 <br />apparent hybrids. <br />Lower Yampa Canyon: Muscle and fin tissue samples were taken from 2 razorback <br />sucker, 30 flannel mouth sucker, 30 bluehead sucker, and I apparent hybrid. Sex products <br />were taken from 2 razorback sucker (I male and I female). <br />Stewart Lake Drain. Old Charley Wash Lower Duchesne: Muscle and fin tissue samples <br />were taken from II razorback sucker. <br /> <br />Lake Powell - An extensive sampling effort was conducted again to locate razorback sucker <br />aggregations in Lake Powell, capture individual fish, apply PIT -tags, take muscle and fin tissue <br />samples, take gamete samples, and then transport them to refuge at Ouray EFCES. USBR, <br />Utah Division of Natural Resources (UDNR), and USFWS participated in this effort. <br />San Juan River Ann: In 1990, II fish were captured. In 1991, 3 fish were captured. <br />Colorado RiverfDirty Devil Ann (}lite Marina): In both 1990 and 1991,4 fish <br />were captured. <br /> <br />Cooperative agreements with ASU, UCLA and USBR were extended. A contract to perform non- <br />lethal tissue sample genetic analysis from several razorback sucker was awarded and was designed <br />to complement the work done at UCLA (U.S. Fish and Wildlife Service 199Ib). By the end of 1991, <br />genetic analysis was completed on the non-lethal tissue sampling project. Mitochondrial DNA <br />analysis was initiated on sex tissues at ASU UCLA began expanding the allozyme systems necessary <br />for analysis of fin and muscle tissues from the same fish. <br /> <br />19 <br />