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<br />002777 <br /> <br />Additionally, in 1992, 42 humpback chubs were sampled from the Grand Canyon, and adequate chub <br />samples were obtained from the Virgin River, Moapa River, and Pahranagat Valley, and Chevalon <br />Creek of the upper Little Colorado system. <br /> <br />A detailed data report providing the sampling and analyses status of each 'population sampled was <br />prepared and issued in the fall of 1992. This report revealed that a total of 1185 specimens had been <br />sampled for correlated genetic and morphological studies of which 867 represented freshly sampled <br />wild populations while 318 were based on captive populations. Seven first generation (F ,) bonytails <br />previously used in non-lethal sampling experiments at Ouray EFCES were also incorporated into the <br />study. Thus, by the end of 1992, all required specimens for sequencing studies of mitochondrial DNA <br />(mtDNA) had been extracted, with the exception of a small number yet to be collected in 1993. <br /> <br />Sampling for chubs of the Gila robusta (roundtail) complex was completed in June, 1993 in all five <br />Mexican drainages (Yaqui, Mayo, Fuerta, Sinaloa, Culiacan) which they are known to inhabit These <br />samples served as a basis for analyzing variation in Colorado Basin members of the complex. Further, <br />other Mexican Qiill species were sampled to serve as comparative genetic material for the complex <br />overall. A total of approximately 200 chubs representing nine populations in six drainages were <br />collected and apportioned for allozyme, mtDNA, and morphological studies. Sampling offive Gila <br />River system tributaries in Arizona was completed in June, 1993 yielding representation of virtually <br />all extant lower basin populations except those on White Mountain Apache lands which were not <br />permitted and had to be deleted from the overall effort. (U.S. Fish and Wildlife Service 1993) <br /> <br />The protein electrophoretic work associated with the allozyme study proceeded on schedule during <br />1993. Genetic Analyses, Inc. completed the screening of about 55 presumptive loci on all of the <br />approximately 1200 live chubs sampled. This resulted in tremendous amounts ofraw data. Much <br />of this data was scored and tabulated. Preliminary analyses of a few loci thus far indicated no fixed <br />differences in upper basin Qiill populations, but further analyses remained before variation could be <br />characterized definitively and compared to morphological variation. (U. S. Fish and Wildlife Service <br />1993) <br /> <br />At Arizona State University (ASU), acquisition of raw morphological data in the form of video- <br />camera images fed into a computer data base was essentially completed. This data base included <br />most of the approximately 1200 newly acquired (via non-lethal or conventional sampling) chub <br />specimens from wild populations and an additional 460 captive specimens to supplement data from <br />these populations or to represent extinct ones (U.S. Fish and Wildlife Service 1993). Data from <br />various measurements from these images were analyzed by multi-variate methods to characterize <br />intra- and inter-population variation. That variation was being compared with genetic information <br />obtained from protein electrophoresis (a1lozyme) and the results were synthesized into the final report <br />concerning morphology (U.S. Fish and Wildlife Service 1995a). The morphometric studies provided <br />evidence to support the hypothesis that the Qiill complex in the Colorado River System contained <br />three distinct species (Wydoski 1996d). <br /> <br />AJlozyme investigations continued in 1994 with the completion of electrophoretic procedures on all <br />samples, including numerous cross-comparison runs. In early 1995, electrophoretic scores for all loci <br /> <br />17 <br />