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<br />0020~1 <br /> <br />for successful spawning, with 160,000 fry shipped to Colorado from 1981 to <br />1988. Hilt frcm wild greenbacks from Como Creek, Hunters Creek, Hidden Valley <br />Creek and the poudre River was also collected and used to fertilize ova from <br />Bozeman females (Dwyer, 1988). This action also helped enhance the genetic <br />diversity of the broodstock. <br /> <br />An attempt was also made to establish a poudre River greenback brood stock at <br />the Saratcga NFH in 1984 and 1985. Eggs collected in 1984 did not survive, <br />but 47\ of the eggs collected in 1985 survived to swim-up. None of the young <br />accepted feed, and all died. Interestingly, eggs from the poudre River <br />population required much less time to develop and hatch than did those of the <br />greenbacks from the Arkansas River drainage's Cascade creek at the Saratoga <br />NFH. At 8 C, eggs from the Poudre River fish required only 16 days to reach <br />the eyed egg stage and 32 days to hatch, compared to 29 days to the eyed stage <br />and 39 days to hatch for eggs from Cascade Creek (J. Hammer, Saratoga NFH, <br />personal communication). <br /> <br />New South Platte and Arkansas greenback broodstock were initiated at the CDOW <br />Experimental Hatchery at Ft. Collins to replace the aging and unfunded USFWS <br />broodstocks. During 1989, a total of 5,419 eggs were collected from Bear <br />Lake, Como creek, Hunters Creek and the poudre River. In 1990, about 200 eggs <br />were collected from Upper Hutcheson Lake. Fish were produced from all areas <br />except the poudre River, and eggs were collected again from the poudre River <br />in July 1992. Eggs were collected from the CDOW Experimental Hatchery <br />broodstock in 1991 and 1992, with problems with asynchronous spawning <br />experienced during 1992. Malachite green cculd not be used to control fungus <br />in 1992, and all the 1989 year class of brood stock did not survive past the <br />spawning season. <br /> <br />Arkansas Drainaae Broodstock. The Greenback Recovery Plan also calls for <br />development of an Arkansas River greenback broodstock. To develop this <br />broodstock, greenbacks from Cascade Creek were introduced into McAlpine Pond <br />(privately owned) and Lytle Pond (on Ft. Carson Army Base). In 1984, eggs <br />were collected from the greenbacks spawning in McAlpine Pond and in Lytle Pond <br />and were sent to Saratcga NFH. Fry and catchable-sized greenbacks were <br />produced at Saratoga NFH from 1987 through 1992. Due to FWS funding problems <br />and the predominance of old adults within the Saratoga broodstock, the <br />Arkansas River broodstcck program was transferred from Saratoga NFH to the <br />CDOW Experimental Hatchery. To facilitate establishment of the new broodstock <br />at the CDOW Experimental Hatchery, 3,200 eggs from South Apache Creek and <br />10,000 eggs from Saratoga NFH were shipped to the CDOW Experimental Hatchery <br />in 1992. Following the collection of eggs at the Saratoga NFH in 1992, the <br />remaining greenbacks at Saratoga NFH were lost due to water problems at the <br />hatchery. <br /> <br />Stockina <br /> <br />A wide range of stocking rates and methods have been used to re-introduce <br />greenbacks into historic'habitats. Early methods usually involved stocking <br />low numbers (64 to 84) of adult and sub-adult greenbacks into renovated <br />habitats during the fall of the year. Only small numbers of greenbacks were <br />stocked due to the limited number of fish available from Como Creek. <br />Unfortunately, colonization of the habitat was slow and genetic diversity was <br />impaired due to the limited numbers of fish stocked. These problems <br />undermined confidence in the ability to establish fishable populations <br /> <br />The captive broodstock programs were initiated to allow more rapid <br />establishment of new populations, to protect the small historic populations <br />from over utilization as brood stock sources and to allow for genetic <br />management. The captive brood stock program produced enough greenback fry to <br />support stocking within each restoration site for at least three consecutive <br /> <br />14 <br />