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Biological Data <br />This segment of the Animas River is a moderate gradient stream with moderate to large substrate <br />size, often punctuated by large boulders within the stream channel. Many portions of the stream <br />channel have been affected by historic mining that moved bedload materials. Natural hydrologic <br />processes since the mining activity ceased have created a natural stream channel, but some <br />portions of the river are still highly braided and lack good width to depth ratios. <br />Water quality in the stream segment is affected by heavy metals from both natural sources and <br />historic mining activities within the watershed. Minnie Gulch and Maggie Gulch contribute <br />water to the river that has lower concentrations of heavy metals, so water quality improves as <br />you move downstream through the reach. <br />Fish surveys have documented naturally reproducing populations of brook trout in this stream <br />reach. Brook trout are the trout species that are most tolerant of heavy metal loads, but they <br />indicate that the stream has sufficient macroinvertebrates to provide for fish forage, and that the <br />stream has basic ecologic functions. Brook trout are not found immediately above this reache, <br />indicating that heavy metal loads in that location are too high for fish habitat. The <br />recommended reach provides an important connectivity between good fish habitat found in <br />Minnie Gulch, Maggie Gulch, and Cunningham Creek. The CWCB has previously appropriated <br />instream flow water rights on all three of these important tributaries, based upon <br />recommendations from BLM and the Colorado Division of Wildlife. <br />The riparian community consists primarily of willows and potentilla. As you move downstream <br />and the water quality improves, the riparian community is more vigorous and has succeeded in <br />converting the stream from a braided system to a channelized environment that is more favorable <br />to fish populations. <br />Field Survey Data <br />BLM staff used the R2Cross methodology to quantify the amount of water required to preserve <br />the natural environment to a reasonable degree. The R2Cross method requires that stream <br />discharge and channel profile data be collected in a riffle stream habitat type. Riffles are most <br />easily visualized, as the stream habitat types that would dry up first should streamflow cease. <br />This type of hydraulic data collection consists of setting up a transect, surveying the stream <br />channel geometry, and measuring the stream discharge. <br />Biological Flow Recommendation <br />The CWCB staff relied upon the biological expertise of the cooperating agencies to interpret <br />output from the R2Cross data collected to develop the initial, biologic instream flow <br />recommendation. This initial recommendation is designed to address the unique biologic <br />requirements of each stream without regard to water availability. Three instream flow hydraulic <br />parameters, average depth, percent wetted perimeter, and average velocity are used to develop <br />biologic instream flow recommendations. The CDOW has determined that maintaining these <br />three hydraulic parameters at adequate levels across riffle habitat types, aquatic habitat in pools <br />and runs will also be maintained for most life stages of fish and aquatic invertebrates (Nehring <br />1979; Espegren 1996). <br />-3- <br />