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Biological Data <br />This segment of Cebolla Creek is a moderate to high gradient stream, with large substrate size. <br />Boulders ranging from one to three feet in diameter in the stream channel are common. <br />Throughout much of the reach, the creek is confined to a narrow canyon. The riparian <br />community is in good condition, but is occasionally impacted by the close proximity of the <br />county road in the canyon. The riparian community is similar to the upper reach, but the willow <br />component of the community is less prominent. The combination of large woody debris and <br />frequent large boulders in the creek channel provide good pool habitat for velocity cover and <br />overwintering. In this reach, riffle habitat appears to be more limited, but it does not appear to be <br />affecting the success of the fish population. Fishery surveys indicate that the creek supports a <br />self - sustaining population of brown trout. The survey revealed a variety of age classes and <br />individual specimens up to 14 inches in length. The Colorado Division of Wildlife has <br />implemented trout habitat improvement projects within this reach. <br />Field Survey Data <br />BLM staff used the R2Cross methodology to quantify the amount of water required to preserve <br />the natural environment to a reasonable degree. The R2Cross method requires that stream <br />discharge and channel profile data be collected in a riffle stream habitat type. Riffles are most <br />easily visualized, as the stream habitat types that would dry up first should streamflow cease. <br />This type of hydraulic data collection consists of setting up a transect, surveying the stream <br />channel geometry, and measuring the stream discharge. <br />Biological Flow Recommendation <br />The CWCB staff relied upon the biological expertise of the cooperating agencies to interpret <br />output from the R2Cross data collected to develop the initial, biologic instream flow <br />recommendation. This initial recommendation is designed to address the unique biologic <br />requirements of each stream without regard to water availability. Three instream flow hydraulic <br />parameters, average depth, percent wetted perimeter, and average velocity are used to develop <br />biologic instream flow recommendations. The CDOW has determined that maintaining these <br />three hydraulic parameters at adequate levels across riffle habitat types, aquatic habitat in pools <br />and runs will also be maintained for most life stages of fish and aquatic invertebrates (Nehring <br />1979; Espegren 1996). <br />For this segment of stream, three data sets were collected with the results shown in Table 1 <br />below. Table 1 shows who collected the data (Party), the date the data was collected (Date), the <br />measured discharge at the time of the survey (Q), the accuracy range of the predicted flows <br />based on Manning's Equation (240% and 40% of Q), the summer flow recommendation based <br />on meeting 3 of 3 hydraulic criteria and the winter flow recommendation based upon 2 of 3 <br />hydraulic criteria. It is believed that recommendations that fall outside of the accuracy range of <br />the model, over 250% of the measured discharge or under 40% of the measured discharge may <br />not give an accurate estimate of the necessary instream flow required. <br />Table 1: Data <br />Party <br />Date <br />Q <br />250 % -40% <br />Summer 3/3 <br />Winter (2/3) <br />BLM <br />10/8/2008 <br />39.80 <br />99.5-15.9 <br />31.73 <br />Out of range <br />BLM <br />10/8/2008 <br />41.85 <br />104.6-16.7 <br />28.72 <br />Out of range <br />-3- <br />