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Biological Data <br />The USFS, in September of 1997, collected stream cross section information, natural <br />environment data, and other data needed to quantify the instream flow needs for this reach of <br />Middle Creek. Middle Creek is classified as a medium stream (between 20 to 35 feet wide) and <br />fishery surveys indicate the stream environment of the Middle Creek supports rainbow trout <br />(Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis). <br />Field Survey Data <br />CDOW staff used the R2Cross methodology to quantify the amount of water required to preserve <br />the natural environment to a reasonable degree. The R2Cross method requires that stream <br />discharge and channel profile data be collected in a riffle stream habitat type. Riffles are most <br />easily visualized, as the stream habitat types that would dry up first should streamflow cease. <br />This type of hydraulic data collection consists of setting up a transect, surveying the stream <br />channel geometry, and measuring the stream discharge. <br />Biological Flow Recommendation <br />The CWCB staff relied upon the biological expertise of the cooperating agencies to interpret <br />output from the R2Cross data collected to develop the initial, biologic instream flow <br />recommendation. This initial recommendation is designed to address the unique biologic <br />requirements of each stream without regard to water availability. Three instream flow hydraulic <br />parameters, average depth, percent wetted perimeter, and average velocity are used to develop <br />biologic instream flow recommendations. The CDOW has determined that maintaining these <br />three hydraulic parameters at adequate levels across riffle habitat types, aquatic habitat in pools <br />and runs will also be maintained for most life stages of fish and aquatic invertebrates (Nehring <br />1979; Espegren 1996). <br />For this segment of stream, one data set was collected with the results shown in Table 1 below. <br />Table 1 shows who collected the data (Party), the date the data was collected (Date), the <br />measured discharge at the time of the survey (Q), the accuracy range of the predicted flows <br />based on Maiming's Equation (240% and 40% of Q), the summer flow recommendation based <br />on meeting 3 of 3 hydraulic criteria and the winter flow recommendation based upon 2 of 3 <br />hydraulic criteria. It is believed that recommendations that fall outside of the accuracy range of <br />the model, over 250% of the measured discharge or under 40% of the measured discharge may <br />not give an accurate estimate of the necessary instream flow required. <br />Table 1: Data <br />Party <br />Date <br />Q <br />250 % -40% <br />Summer (3/3) <br />Winter (2/3) <br />USFS <br />9/5/1997 <br />2.8 <br />7.1 — 1.1 <br />1 5.1 <br />2.8 <br />The summer flow recommendation, which met 3 of 3 criteria and is within the accuracy range of <br />the R2CROSS model is 5.1 cfs. The winter flow amount, which meets 2 of 3 criteria, is 2.8 cfs. <br />Hydrologic Data and Analysis <br />After receiving the cooperating agency's biologic recommendation, the CWCB staff conducted <br />an evaluation of the stream hydrology to determine if water was physically available for an <br />instream flow appropriation. This evaluation was done through a computation that is, in essence, <br />-3- <br />