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Evaluating the impacts of changing the lower limit of discharge on biological resources also was raised <br />in the EA. <br />A 2008 biological opinion (BO) released by the U.S. Fish and Wildlife Service (U.S. Fish and Wildlife <br />Service, 2008) called for two conservation measures that are addressed in this plan in response to the <br />experimental flows. The conservation measures under the 2008 BO include the nearshore ecology <br />project and a study of the impacts of flow transition, that is, the impacts of the flows fluctuating in <br />August followed by reduced, steady flows in September during the experimental period. The BO <br />suggested that the long -term impacts of the experimental flows would benefit humpback chub by <br />creating and improving rearing habitats and increasing growth and survival. The BO also calls for <br />monitoring the impacts of the March 2008. HFE and the fall steady flows on humpback chub. <br />There was not sufficient time to prepare this plan and implement the activities described herein during <br />the 2008 FSEF; the GCMRC and its cooperators were preparing to undertake a substantial body of <br />research associated with the March 2008 high -flow experiment (HFE) in February when the EA was <br />released. However, there were ongoing studies during the 2008 FSEF, including a pilot project to start <br />collecting data for the nearshore ecology project. Additionally, select biological resources were studied <br />in association with the March 2008 HFE, and final reporting for all projects is nearly complete (Melis <br />and others, 2010). Some baseline data from these studies are available and will be incorporated into the <br />longer term studies. <br />Previous Experiments and Studies <br />Stable and low discharge is expected to benefit native fish in the Colorado River below Glen Canyon <br />Dam by improving the quality of nearshore habitats that are occupied by juvenile life stages of native <br />fish. Warming of nearshore water temperatures, stability of nearshore environments, and increases in <br />food resources are the main habitat parameters that are hypothesized to improve with stable and low <br />discharge. A number of these hypotheses have been investigated and the findings are summarized <br />below. <br />Nearshore Water Temperature <br />The water temperature of nearshore habitats in the Colorado River is influenced by a variety of factors, <br />especially the temperature of releases, air temperatures, the residence time of water in the mainstem, and <br />the residence time of water in the nearshore environment (Korman and others, 2006; Wright and others, <br />2008). The elevation of Lake Powell and the thickness of the epilimnion have a major influence on <br />release temperatures. In particular, the temperature of water discharged from Glen Canyon Dam during <br />the summer of 2005 was 4 °C warmer relative to the summertime average from 1989 to 2000 because of <br />low Lake Powell elevations. The mainstem water temperatures observed between Glen Canyon Dam <br />and the Little Colorado River in 2005 were warmer than temperatures observed in 2000, despite <br />fluctuating flows in 2005 and steady flows in 2000, because of the lower elevation of Lake Powell in <br />2005 (B. Ralston, U.S. Geological Survey, written commun., 2009). <br />